{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:34:28Z","timestamp":1761100468165},"reference-count":5,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,4,12]],"date-time":"2001-04-12T00:00:00Z","timestamp":987033600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[2001,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Circulating hematopoietic progenitor cells (HPCs) are routinely measured by flow cytometry using CD34 expression. As an alternative, the \u201cimmature information\u201d (IMI) channel measurement of the automated hematology analyzer Sysmex SE machines was developed. We tested the IMI channel HPC method with umbilical cord blood specimens. The IMI\u2010HPCs were compared with CD34 counts and numbers of colony\u2010forming units (CFUs). The IMI\u2010HPC data were reproducible and dilution experiments yielded a log\u2010linear relationship. The mean percentage of CD34<jats:sup>+<\/jats:sup> cells in 50 umbilical cords was 0.43 versus 0.11 of HPCs in the IMI channel (correlation coefficient <jats:italic>r<\/jats:italic> = 0.67). Absolute numbers yielded 96.79 \u00d7 10<jats:sup>6<\/jats:sup>\/L CD34<jats:sup>+<\/jats:sup>, 33.17 \u00d7 10<jats:sup>6<\/jats:sup>\/L IMI\u2010HPC, and 35.04 \u00d7 10<jats:sup>6<\/jats:sup>\/L CFU\u2010HPC. Receiver operating characteristics curves were made at various cutoff levels for CD34<jats:sup>+<\/jats:sup> cells to visualize sensitivity and specificity profiles. With median values of 13.56 \u00d7 10<jats:sup>6<\/jats:sup>\/L for IMI\u2010HPC and 20 \u00d7 10<jats:sup>6<\/jats:sup>\/L for CD34<jats:sup>+<\/jats:sup> as cutoff points (the levels used in the laboratory to start stem cell pheresis), the percentage of false\u2010negative observations was 70.4%. To exclude the influence of storage time, tests were repeated until 72 h after umbilical cord collection. Total white blood cell count decreased in most cases, whereas absolute number of IMI\u2010HPC tended to increase in time. In conclusion, if HPC measurements in the IMI channel are used to monitor circulating stem cells during mobilization, one has to be aware of a very low correlation between these results and those of other methods such as CD34<jats:sup>+<\/jats:sup> analysis and colony growth. False\u2010negative results do occur, but if events are seen in the IMI channel, this simple monitoring technique is useful to predict the presence of circulating stem cells. Cytometry (Comm. Clin. Cytometry) 46:114\u2013118, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.1073","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T20:23:10Z","timestamp":1030306990000},"page":"114-118","source":"Crossref","is-referenced-by-count":8,"title":["Hemopoietic stem and precursor cell analysis in umbilical cord blood using the sysmex SE\u20109000 IMI channel"],"prefix":"10.1002","volume":"46","author":[{"given":"R.","family":"Kraai","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. G. F.","family":"Reymer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. G.","family":"Brouwer\u2010Mandema","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. M.","family":"van Beckhoven","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Hoogeboom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"le Cessie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. C.","family":"Kluin\u2010Nelemans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,12,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1999.01584.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1159\/000040887"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1702015"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19970615)30:3<109::AID-CYTO1>3.0.CO;2-G"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990090512"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.1073","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.1073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T23:05:56Z","timestamp":1696979156000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.1073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,4,15]]},"references-count":5,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,4,15]]}},"alternative-id":["10.1002\/cyto.1073"],"URL":"https:\/\/doi.org\/10.1002\/cyto.1073","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,4,15]]}}}