{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:27:56Z","timestamp":1791282476929,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,8,14]],"date-time":"2001-08-14T00:00:00Z","timestamp":997747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Hungarian National Science Foundation","award":["022429"],"award-info":[{"award-number":["022429"]}]},{"name":"Hungarian National Science Foundation","award":["032584"],"award-info":[{"award-number":["032584"]}]},{"name":"Hungarian National Academy of Sciences","award":["97-106"],"award-info":[{"award-number":["97-106"]}]},{"name":"Hungarian Ministry of Health","award":["320\/96"],"award-info":[{"award-number":["320\/96"]}]},{"name":"Hungarian Ministry of Health","award":["58\/2000"],"award-info":[{"award-number":["58\/2000"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[2001,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Characteristic genetic changes underlying the metastatic progression of malignant melanoma is incompletely understood. The goal of our study was to explore specific chromosomal alterations associated with the aggressive behavior of this neoplasm. Comparative genomic hybridization was performed to screen and compare genomic imbalances present in primary and metastatic melanomas. Sixteen primary and 12 metastatic specimens were analyzed. We found that the pattern of chromosomal aberrations is similar in the two subgroups; however, alterations present only in primary and\/or metastatic tumors were also discovered. The mean number of genetic changes was 6.3 (range 1\u201314) in primary and 7.8 (range 1\u201316) in metastatic lesions. Frequent losses involved 9p and 10q, whereas gains most often occurred at 1q, 6p, 7q, and 8q. Distinct, high\u2010level amplifications were mapped to 1p12\u2013p21 and 1p22\u2013p31 in both tumor types. Amplification of 4q12\u2013q13.1, 7q21.3\u2010qter and 8q23\u2010qter were detected only in primary tumors. The 20q13\u2010qter amplicon was present in a metastatic tumor. The number of genetic alterations were significantly higher in primary tumors which developed metastases within one year after the surgery compared to tumors without metastasis during this time period. Fluorescence in situ hybridization with centromeric and locus\u2010specific probes was applied to validate CGH results on a subset of tumors. Comparison of FISH and CGH data gave good correlation. The aggressive behavior of melanoma is associated with accumulation of multiple genetic alterations. Chromosome regions, which differ in the primary and metastatic lesions, may represent potential targets to identify metastases\u2010related chromosomal alterations. Cytometry (Comm. Clin. Cytometry) 46:222\u2013232, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.1131","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:04:02Z","timestamp":1030309442000},"page":"222-232","source":"Crossref","is-referenced-by-count":90,"title":["Chromosomal imbalances in primary and metastatic melanomas revealed by comparative genomic hybridization"],"prefix":"10.1002","volume":"46","author":[{"given":"Margit","family":"Bal\u00e1zs","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsuzsa","family":"\u00c1d\u00e1m","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Treszl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1gnes","family":"B\u00e9g\u00e1ny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e1nos","family":"Hunyadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00f3za","family":"\u00c1d\u00e1ny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,12,26]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2744(199811)23:3<132::AID-MC2>3.0.CO;2-1"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.135.3.275"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/24.5.897"},{"key":"e_1_2_5_5_2","first-page":"193","article-title":"Prognostic factors in 1,521 melanoma patients with distant metastases","volume":"181","author":"Barth A","year":"1995","journal-title":"J Am Coll Surg"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000164143"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/00008390-199910000-00001"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(95)00057-V"},{"key":"e_1_2_5_9_2","first-page":"4487","article-title":"A malignant melanoma tumor suppressor on human chromosome 11","volume":"56","author":"Robertson G","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_5_10_2","first-page":"2527","article-title":"Isolation and characterization of genes associated with chromosome\u20106 mediated tumor suppression in human malignant melanoma","volume":"12","author":"Ray ME","year":"1996","journal-title":"Oncogene"},{"key":"e_1_2_5_11_2","first-page":"516","article-title":"Functional evidence of novel tumor suppressor genes for cutaneous malignant melanoma","volume":"59","author":"Parris CN","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201470"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6109(05)70513-2"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.12.4614"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8153634"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8023167"},{"key":"e_1_2_5_17_2","first-page":"1107","article-title":"DNA copy number amplifications in human neoplasms: review of comparative genomic hybridization studies","volume":"152","author":"Knuutila S","year":"1998","journal-title":"Am J Pathol"},{"issue":"3","key":"e_1_2_5_18_2","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1016\/S0002-9440(10)65166-8","article-title":"DNA copy number losses in human neoplasms","volume":"155","author":"Knuutila S","journal-title":"Am J Pathol"},{"key":"e_1_2_5_19_2","first-page":"3954","article-title":"Direct visualization of the clonal progression of primary cutaneous melanoma application of tissue microdissection and comparative genomic hybridization","volume":"55","author":"Wiltshire RN","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_5_20_2","first-page":"1270","article-title":"Chromosomal gains and losses in primary cutaneous melanomas detected by comparative genomic hybridization","volume":"58","author":"Bastian BC","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_5_21_2","first-page":"1968","article-title":"Gene amplifications characterize acral melanoma and permit the detection of occult tumor cells in the surrounding skin","volume":"60","author":"Bastian BC","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_5_22_2","volume-title":"Molecular cloning. A laboratory manual","author":"Sambrock J"},{"key":"e_1_2_5_23_2","first-page":"3555","article-title":"Genetic alterations in primary bladder cancers and their metastases","volume":"58","author":"Hovey RM","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401978"},{"key":"e_1_2_5_25_2","first-page":"342","article-title":"Genetic changes in primary and recurrent prostate cancer by comparative genomic hybridization","volume":"55","author":"Visakorpi T","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199803)21:3<177::AID-GCC1>3.0.CO;2-X"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(98)00213-1"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199706)19:2<84::AID-GCC3>3.0.CO;2-W"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(98)00159-9"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870100402"},{"key":"e_1_2_5_31_2","first-page":"3817","article-title":"Chromosomal gains and losses in uveal melanomas detected by comparative genomic hybridization","volume":"54","author":"Speicher MR","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.2000.1535"},{"key":"e_1_2_5_33_2","first-page":"97","article-title":"c\u2010MYC and nodular malignant melanoma","volume":"89","author":"Greulich KM","year":"2000","journal-title":"A case report. Cancer"},{"key":"e_1_2_5_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01534687"},{"key":"e_1_2_5_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-0960.1999.00310.x"},{"key":"e_1_2_5_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2264(199708)19:4<278::AID-GCC11>3.0.CO;2-C"},{"key":"e_1_2_5_37_2","first-page":"516","article-title":"Functional evidence of novel tumor suppressor genes for cutaneous malignant melanoma","volume":"59","author":"Parris CN","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_5_38_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19970620)74:3<255::AID-IJC4>3.0.CO;2-Y"},{"key":"e_1_2_5_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004390050642"},{"key":"e_1_2_5_40_2","first-page":"883","article-title":"Differences in chromosomal aberrations between nodular and superficial spreading malignant melanoma detected by interphase cytogenetics","volume":"78","author":"Poetsch M","year":"1998","journal-title":"Lab Invest"},{"key":"e_1_2_5_41_2","first-page":"3660","article-title":"Disruption of the MMAC1\/PTEN gene by deletion or mutation is a frequent event in malignant melanoma","volume":"57","author":"Guldberg P","year":"1997","journal-title":"Cancer Res"},{"key":"e_1_2_5_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(97)00016-2"},{"key":"e_1_2_5_43_2","doi-asserted-by":"crossref","first-page":"3137","DOI":"10.1016\/0165-4608(88)90200-2","article-title":"Possible involvement of the chromosome region 10q24\u2013q26 in early stages of melanocytic neoplasia","volume":"30","author":"Parmiter AH","year":"1988","journal-title":"Cancer Genet Cytogenet"},{"key":"e_1_2_5_44_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870080307"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.1131","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.1131","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T06:22:20Z","timestamp":1697437340000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.1131"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,8,15]]},"references-count":43,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,8,15]]}},"alternative-id":["10.1002\/cyto.1131"],"URL":"https:\/\/doi.org\/10.1002\/cyto.1131","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,8,15]]}}}