{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:54:39Z","timestamp":1788872079403,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,8,14]],"date-time":"2001-08-14T00:00:00Z","timestamp":997747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005970","name":"Deutsche Stiftung f\u00fcr Herzforschung","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005970","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005971","name":"Deutsche Herzstiftung","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005971","id-type":"DOI","asserted-by":"crossref"}]},{"name":"S\u00e4chsisches Ministerium f\u00fcr Wissenschaft und Kunst"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[2001,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Postoperative edema and effusion (POEE) following cardiopulmonary bypass (CPB) surgery in children retards recovery and may aggravate postpericardiotomy (PPS), capillary leak syndrome (CLS), or multiorgan failure (MOF). Compared with complication\u2010free children, POEE affected children have different preoperative serum levels of circulating cytokines and adhesion molecules. These levels may be used preoperatively to assess POEE, but their determination is time consuming, costly, and a substantial blood volume is required. Altered serum levels of cytokines and adhesion molecules also may be reflected in altered antigen expression on circulating blood leukocytes. The predictive potential of flow cytometric (FCM) leukocyte immunophenotyping was explored as a sensitive and fast method that required small blood samples. Blood samples taken 24 h preoperatively from 49 patients (3\u201318 years old) were stained with monoclonal antibodies for adhesion molecules (ICAM\u20101, LFA\u20101, Mac\u20101) or constitutive\/activation markers (CD4, CD14, CD16, CD25, CD54, CD69, HLA\u2010DR) and measured on a microbead calibrated FCM. Neutrophils, monocytes, and eosinophils from POEE patients express higher preoperative levels of LFA\u20101, monocytes, HLA\u2010DR, and other activation markers (all\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.03). Over 89% of the patients were classified correctly by using two discriminant analysis methods (sensitivity, &gt;76%; specificity, &gt;86%; positive prediction, &gt;80%; negative prediction, &gt;83%). Granulocytes and monocytes of postoperative POEE patients exhibit significant preoperative immune activation, suggesting an increased risk for patients with atopic\/allergic predisposition. Surgical trauma and CPB cause additional immune activation, leading to POEE by a summative response. Most patients at risk for POEE can be identified preoperatively by using data pattern analysis on FCM\u2010derived parameters. Cytometry (Comm. Clin. Cytometry) 46:247\u2013253, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/cyto.1135","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T17:04:02Z","timestamp":1030295042000},"page":"247-253","source":"Crossref","is-referenced-by-count":26,"title":["Preoperative prediction of postoperative edema and effusion in pediatric cardiac surgery by altered antigen expression patterns on granulocytes and monocytes"],"prefix":"10.1002","volume":"46","author":[{"given":"Attila","family":"T\u00e1rnok","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f3zsef","family":"Bocsi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Pipek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel","family":"Osmancik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00fcnter","family":"Valet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Schneider","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Hambsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,12,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(98)00484-7"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002469900417"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(99)00093-4"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-1013820"},{"key":"e_1_2_6_6_2","first-page":"13","article-title":"Capillary leak syndrome after open heart surgery for congenital heart defects: therapy with C1\u2010inhibitor","volume":"9","author":"Stieh J","year":"1996","journal-title":"Biomed Prog"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01780757"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.99.18.2378"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-199902000-00041"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1010-7940(98)00062-1"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001340050600"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.95.8.2027"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(00)70231-5"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.11.4182"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/hrt.75.3.287"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI6872"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.98.12051094"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1997.tb01194.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1053-0770(99)90210-0"},{"key":"e_1_2_6_20_2","first-page":"845","volume-title":"Immunological and serological discrimination of children with and without post\u2010surgical capillary leak syndrome","author":"T\u00e1rnok A","year":"1997"},{"key":"e_1_2_6_21_2","series-title":"Methods in cell biology","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/S0091-679X(01)64026-3","author":"Valet G","year":"2001"},{"key":"e_1_2_6_22_2","first-page":"54","article-title":"Clinical significance of neutrophil adhesion molecules expression after coronary angioplasty on the development of restenosis","volume":"70","author":"Inoue T","year":"1998","journal-title":"Thromb Haemost"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1998.00626.x"},{"key":"e_1_2_6_24_2","first-page":"364","article-title":"Differential patterns of neutrophil adhesion molecules during CPB in humans","volume":"94","author":"Gali\u00f1anes M","year":"1996","journal-title":"Circulation"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.3109\/14017439709058075"},{"key":"e_1_2_6_26_2","volume-title":"Durchflusszytometrie in der klinischen Zelldiagnostik","author":"Schmitz G","year":"1994"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19981001)33:2<166::AID-CYTO11>3.0.CO;2-S"},{"key":"e_1_2_6_28_2","first-page":"B1","volume-title":"SPSS\/PC+. Advanced statistics TM","author":"Norussis MJ","year":"1988"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/1053-0770(94)90165-1"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(98)70335-6"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/11.1.1"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1529-1049(01)00033-2"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03013433"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.163.9.5029"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1006\/clim.1999.4775"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.158.1.367"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199911)29:11<3571::AID-IMMU3571>3.0.CO;2-V"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/35037026"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.1135","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.1135","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T02:22:46Z","timestamp":1697422966000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.1135"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,8,15]]},"references-count":37,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,8,15]]}},"alternative-id":["10.1002\/cyto.1135"],"URL":"https:\/\/doi.org\/10.1002\/cyto.1135","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,8,15]]}}}