{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:25:55Z","timestamp":1761056755641},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2001,10,26]],"date-time":"2001-10-26T00:00:00Z","timestamp":1004054400000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[2001,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The search continues to find methods to more effectively distinguish colorectal carcinoma patients who could be separated into high\u2010risk and low\u2010risk categories. Investigators have reported on the detection of occult micrometastases in bone marrow using antibodies to cytokeratin, which is a marker of epithelial cells but which has no tissue specificity, as opposed to villin, a cytoskeletal protein that is specifically involved in the formation of brush\u2010border microvilli in the small intestine and colon epithelium.<\/jats:p><jats:p>Specificity and sensitivity of antibody to villin (ID2C3) and antibody to cytokeratin (A45\u2010B\/B3) were first studied in normal bone marrow and in a test system in which cancer cell lines were mixed in normal bone marrow. In a preliminary study including 16 colorectal carcinoma patients, we compared the number of villin\u2010positive cells with cytokeratin\u2010presenting cells.<\/jats:p><jats:p>As A45\u2010B\/B3, ID2C3 was determined to be sensitive enough to detect one cancer cell in 10<jats:sup>6<\/jats:sup> hematopoietic cells. Staining of hematopoietic cells with irrelevant antibody and a light staining of megakaryocytes with ID2C3 limited the specificity of the method. In colorectal carcinoma patients, correlation between ID2C3 and A45\u2010B\/B3 was 94%.<\/jats:p><jats:p>Sensitivity and specificity of ID2C3 antibody to villin were satisfactory. Its clinical relevance must be investigated in further studies. Cytometry (Comm. Clin. Cytometry) 46:281\u2013289, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.1169","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:17:12Z","timestamp":1030310232000},"page":"281-289","source":"Crossref","is-referenced-by-count":2,"title":["Immunocytochemical detection of bone marrow micrometastases in colorectal carcinoma patients, using a monoclonal antibody to 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