{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T14:12:16Z","timestamp":1780927936293,"version":"3.54.1"},"reference-count":5,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,3,25]],"date-time":"2005-03-25T00:00:00Z","timestamp":1111708800000},"content-version":"vor","delay-in-days":8790,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1981,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Flow sorters produce microdroplets as part of their normal operation, and if these microdroplets escape into the room, they are potentially hazardous. We have tested several aerosol\u2010control measures on a commercial flow sorter. To accomplish this, T\u20104 phages were introduced into the sorter's liquid jet through the sample injection tube, and culture plates containing lawns of T\u20104\u2010sensitive <jats:italic>Escherichia coli<\/jats:italic> bacteria were exposed around the sorter for each operational configuration. After the exposure, the plates were incubated and then scored for plaques. A single phage\u2010containing microdroplet landing on a plate was sufficient to cause a plaque of lysed bacteria to form. The number of plaques was thus an indicator of how much aerosol was released for each configuration. Aerosols were controlled most effectively by catching the central, undeflected stream in a vacuum\u2010exhausted tube; this technique, coupled with the manufacturer's vacuum\u2010exhaustion of the air around the sorting location, produced no plaques. Several failure modes were tested, including having the central stream hit the outside of the catch\u2010tube or deflection plates, and loss of the manufacturer's vacuum\u2010exhaustion system. Many flow sorters allow the undeflected stream to splash into a flask or beaker, an approach that produces the most plaques if instrument\u2010failure modes are excluded. The simple addition of a catch\u2010tube removed this major contributor to the aerosol production.<\/jats:p>","DOI":"10.1002\/cyto.990010507","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T14:18:50Z","timestamp":1123510730000},"page":"342-345","source":"Crossref","is-referenced-by-count":26,"title":["Evaluation of selected aerosol\u2010control measures on flow sorters"],"prefix":"10.1002","volume":"1","author":[{"given":"John T.","family":"Merrill","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"484","DOI":"10.1128\/am.29.4.484-490.1975","article-title":"Biohazards assessment in large\u2010scale zonal centrifugation","volume":"29","author":"Baldwin CL","year":"1975","journal-title":"Appl Microbiol"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/scientificamerican0376-108"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.905822"},{"key":"e_1_2_1_5_2","volume-title":"Flow Cytometry and Sorting","author":"Melamed MR","year":"1979"},{"key":"e_1_2_1_6_2","first-page":"46","volume-title":"Experiments in Molecular Genetics","author":"Miller JH","year":"1972"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990010507","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990010507","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T16:01:37Z","timestamp":1699804897000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990010507"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,3]]},"references-count":5,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1981,3]]}},"alternative-id":["10.1002\/cyto.990010507"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990010507","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,3]]}}}