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Complex structures composed of overlapping colonies could be reduced to the consitituent single colonies by special correction programs. Eighteen experiments were evaluated, and the D<jats:sub>0<\/jats:sub> values for colony\u2010forming ability were determined. A systematic but correctable deviation between visual and automated counting was found. The source of this deviation is described and a solution for correction is provided. The automatic procedure reduced the evaluating time by a factor of five to ten.<\/jats:p>","DOI":"10.1002\/cyto.990060208","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T16:02:08Z","timestamp":1123516928000},"page":"130-136","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of colony\u2010forming ability experiments using normal and DNA repair\u2010deficient human fibroblast strains and an automatic colony counter"],"prefix":"10.1002","volume":"6","author":[{"given":"Heinz Walter","family":"Thielmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Hagedorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Werner","family":"Freber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.4.1984"},{"key":"e_1_2_1_3_2","first-page":"926","article-title":"Survey of radiosensitivity in a variety of human cell strains","volume":"40","author":"Arlett CF","year":"1980","journal-title":"Cancer Res"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12524886"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(71)90276-5"},{"key":"e_1_2_1_6_2","volume-title":"From image to analysis: Object detection and measurement","author":"Bender MK","year":"1984"},{"key":"e_1_2_1_7_2","unstructured":"BrusickDJ: Automated plate\u2010counting assays in the AmesSalmonella\/microsome mutagenicity test.Pharmaceutical Technology January:37\u201341 1978."},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.27.1.264-267.1974"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990030609"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1214\/aoms\/1177704172"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01538669"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(80)90163-5"},{"key":"e_1_2_1_13_2","first-page":"179","volume-title":"Cloning of Human Tumor Stem Cells","author":"Kressner BE","year":"1980"},{"key":"e_1_2_1_14_2","first-page":"904","article-title":"Repair of ultraviolet light damage in a variety of human fibroblast cell strains","volume":"37","author":"Lehmann AR","year":"1977","journal-title":"Cancer Res"},{"key":"e_1_2_1_15_2","first-page":"1743","article-title":"The enzymatic reduction of the N\u2010hydroxy derivatives of 2\u2010acetylaminofluorene and related carcinogens by tissue preparations","volume":"25","author":"Lotlikar PD","year":"1965","journal-title":"Cancer Res"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(66)90026-9"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/54.6.1287"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/258760a0"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(83)90054-4"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/10420157908201738"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0191-278X(81)90004-4"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00406246"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910310603"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990060208","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990060208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T15:45:20Z","timestamp":1697816720000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990060208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,3]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1985,3]]}},"alternative-id":["10.1002\/cyto.990060208"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990060208","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,3]]}}}