{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T11:24:33Z","timestamp":1789903473272,"version":"4.0.1"},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,3,30]],"date-time":"2005-03-30T00:00:00Z","timestamp":1112140800000},"content-version":"vor","delay-in-days":7212,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1985,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The study was made using a rat model for human acute myelocytic leukemia (BNML), which shows striking similarities with human acute myelocytic leukemia (AML). A monoclonal antibody (MCA\u2010Rm124), raised against BNML cells, allowed the recognition of the leukemic cell fraction. The discriminative capacity of the monoclonal antibody is based on differences in labeling intensities between normal and leukemic cells. After i.v. cellular transfer of leukemia, the growth of the leukemic cell population in the bone marrow, the liver, and the spleen was monitored using MCA\u2010Rm124 and flow cytometry. For the bone marrow and the liver, a clonogenic assay for leukemic cells was used to quantify the cell content in these organs. A good correlation was found between the bioassay\u2010derived data and the flow\u2010cytometry\u2010derived data. The doubling times of the leukemic cell population were not equal for the two organs studied, indicating that a number of different processes contribute to the net cell production per organ.<\/jats:p><jats:p>Apart from their application in the detection of residual leukemia, monoclonal antibodies might be employed in the analysis of the growth kinetics of the \u201cinvisible\u201d leukemic cell population.<\/jats:p>","DOI":"10.1002\/cyto.990060411","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T16:07:03Z","timestamp":1123517223000},"page":"342-347","source":"Crossref","is-referenced-by-count":23,"title":["Detection of minimal disease in acute leukemia using flow cytometry: Studies in a rat model for human acute leukemia"],"prefix":"10.1002","volume":"6","author":[{"given":"Anton C. M.","family":"Martens","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton","family":"Hagenbeek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1182\/blood.V61.4.628.628","article-title":"Selective expression of the common acute lymphoblastic leukemia (gp100) antigen on immature lymphoid cells and their malignant counterparts","volume":"61","author":"Greaves M","year":"1983","journal-title":"Blood"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(77)90001-7"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-5670-4_3"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(81)90072-2"},{"key":"e_1_2_1_6_2","first-page":"361","article-title":"Functional cell compartments in a rat model for human acute myelocytic leukemia","volume":"12","author":"Hagenbeek A","year":"1979","journal-title":"Cell Tissue Kinet"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.157.1.114"},{"key":"e_1_2_1_8_2","first-page":"172","article-title":"The treatment of acute myelogenous leukemia in adults","volume":"19","author":"Lister TA","year":"1982","journal-title":"Semin Hematol"},{"key":"e_1_2_1_9_2","first-page":"667","article-title":"Characteristics of a monoclonal antibody (Rm124) against acute myelocytic leukemia cells","volume":"12","author":"Martens ACM","year":"1984","journal-title":"Exp Hematol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990020105"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.154.4.1249"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-25807-1_1"},{"key":"e_1_2_1_13_2","first-page":"565","article-title":"Relevance of the BN leukemia as a model for human acute myeloid leukemia","volume":"3","author":"Van Bekkum DW","year":"1977","journal-title":"Blood Cells"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-5670-4_2"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990060411","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990060411","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T17:34:58Z","timestamp":1697823298000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990060411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,7]]},"references-count":13,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1985,7]]}},"alternative-id":["10.1002\/cyto.990060411"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990060411","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,7]]}}}