{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:39:46Z","timestamp":1761100786514},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":7067,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1985,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A method is modified to determine the DNA synthesizing cells in primary human breast tumors and cells with idiopathic thrombocytopenic purpura (ITP) with FITC\u2010conjugated monoclonal antibody against bromodeoxyuridine (FITC\u2010M\u2010anti\u2010BrdUrd) and fluorescence microscopy. The DNA synthesizing cells were also determined from a portion of the same tissues by classical tritiated thymidine labeling (<jats:sup>3<\/jats:sup>HdThd) and autoradiography. The results from bromodeoxyuridine labeling index (BrdUrd\u2010LI) and tritiated thymidine labeling index (<jats:sup>3<\/jats:sup>HdThd\u2010LI) obtained from the same tissues were compared. The mean BrdUrd\u2010LI for breast tumor was 5.4 \u00b1 1.0% and the mean <jats:sup>3<\/jats:sup>HdThd\u2010LI was 5.5 \u00b1 1.1%. Similarly, the labeling indexes obtained from mononuclear leukocytes of healthy donors had means of 0.5 \u00b1 0.1% and 0.6 \u00b1 0.1% for BrdUrd\u2010LI and <jats:sup>3<\/jats:sup>HdThd\u2010LI, respectively. The change in the proliferation rate of mononuclear leukocyte population in the samples obtained from patients with ITP could be observed by both methods. The mean BrdUrd\u2010LI of mononuclear leukocytes for this hematological disorder was 5.4 \u00b1 0.9% and the mean <jats:sup>3<\/jats:sup>HdThd\u2010LI was 6.1 \u00b1 0.8%. These results suggest that this relatively simple technique offers an alternative method for determining the DNA synthesizing cells in a given cell population.<\/jats:p>","DOI":"10.1002\/cyto.990060615","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T14:56:33Z","timestamp":1123512993000},"page":"597-601","source":"Crossref","is-referenced-by-count":24,"title":["The use of FITC\u2010conjugated gated monoclonal antibodies for determination of S\u2010phase cells with fluorescence microscopy"],"prefix":"10.1002","volume":"6","author":[{"given":"Nurten","family":"Gunduz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"188","volume-title":"Clinical Laboratory Methods","author":"Bauer JD","year":"1974"},{"key":"e_1_2_1_3_2","first-page":"4","article-title":"Cell\u2010cycleanalysis using a monoclonal antibody to BrdUrd","volume":"17","author":"Dean PN","year":"1984","journal-title":"Cell Tissue Kinet"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.18.5573"},{"key":"e_1_2_1_5_2","first-page":"673","article-title":"Monoclonal anti\u2010bromode\u2010oxyuridine\u2014a powerful reagent for cytokinetic study in\u2010vitro and in\u2010vivo","volume":"17","author":"Dolbeare F","year":"1984","journal-title":"Cell Tissue Kinet"},{"key":"e_1_2_1_6_2","first-page":"1488","article-title":"Influence of the interval between primary tumor removal and chemotherapy on kinetics and growth of metastases","volume":"43","author":"Fisher B","year":"1983","journal-title":"Cancer Res"},{"key":"e_1_2_1_7_2","first-page":"1545","article-title":"Further observations on the inhibition of tumor growth by Corynebacterium Parvum with cyclophosphamide X. 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The Johns Hopkins University School of Medicine","author":"Lehninger AL","year":"1971"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198404123101518"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990060615","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990060615","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T14:54:51Z","timestamp":1694616891000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990060615"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,11]]},"references-count":16,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1985,11]]}},"alternative-id":["10.1002\/cyto.990060615"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990060615","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,11]]}}}