{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T01:20:44Z","timestamp":1707268844066},"reference-count":6,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":7006,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple circuit is described for generating a variety of electronic pulses to test hardware and software for slit\u2010scan chromosome analysis in a flow cytometer. The pulse shape can be changed to have different numbers of local minima, thereby simulating fluorescence pulses from acrocentric, monocentric, and dicentric chromosomes. Long pulses simulate aggregates of chromosomes. The pulse repetition rate as well as the pulse amplitude is variable. Although the circuitry is built with only three integrated circuits, the pulse\u2010to\u2010pulse variation in shape and height is quite small. After digitization of the analog signals, the constructed histograms of pulse integrals show a relative coefficient of variation below 1%. This signal generator provides a valuable tool for a number of electronic test applications that would otherwise require expensive standard particles analyzed in a well\u2010tuned flow cytometer.<\/jats:p>","DOI":"10.1002\/cyto.990070115","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T13:50:31Z","timestamp":1123509031000},"page":"98-100","source":"Crossref","is-referenced-by-count":3,"title":["A pulse generator simulating slit\u2010scan chromosome analysis signals"],"prefix":"10.1002","volume":"7","author":[{"given":"H.\u2010U. L.","family":"Weier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. G.","family":"Eisert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"108","volume-title":"Das TTL\u2010Kochbuch","author":"Astabile Schaltungen","year":"1972"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/27.1.86570"},{"key":"e_1_2_1_4_2","first-page":"249","volume-title":"Flow Cytometry IV","author":"Laerum OD","year":"1980"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/27.1.374608"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(73)90280-2"},{"key":"e_1_2_1_7_2","unstructured":"WeierH EisertWG:A two parameter data acquisition system for high\u2010speed slit\u2010scan analysis of mammalian chromosomes. In preparation"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990070115","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990070115","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T19:44:07Z","timestamp":1697831047000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990070115"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,1]]},"references-count":6,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1986,1]]}},"alternative-id":["10.1002\/cyto.990070115"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990070115","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,1]]}}}