{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T13:19:37Z","timestamp":1775308777280,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":6702,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1986,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Flow cytometry analysis was applied to swine chromosomes prepared from phytohemagglutinin (PHA) stimulated peripheral blood lymphocytes. Flow karyotypes from both sexes and from t(3;7) translocation carrier females were obtained. A certain number of chromosome pairs could be assigned to various peaks. In fact, 13 peaks were observed for 18 autosomal pairs plus X and Y. Moreover, abnormalties owing to the t(3;7) translocation were readily observable.<\/jats:p><jats:p>The number of base pairs for chromosomes associated with the various peaks was estimated by comparison with human flow karyotypes.<\/jats:p><jats:p>The following four peaks were thus sorted: (1) the peak assumed to represent the translocated chromosome 7 plus the normals associated with it; (2) the corresponding peak from a normal swine; (3) the peak assumed to contain among others the normal chromosome 7; and finally (4) the peak corresponding to swine chromosome 1.<\/jats:p><jats:p>Chromosomes of each peak were collected on Pall Biodyne membrane. Following appropriate denaturation and prehybridization, the four samples were hybridized with a human leucocyte antigen (HLA) class I<jats:sup>32<\/jats:sup>P\u2010labelled cDNA probe, representing most of the coding sequence of the HLA B7 gene. The results confirmed previous data from other techniques that assigned the swine MHC(SLA) to chromosome 7.<\/jats:p><jats:p>Subsequently, sorted samples were hybridized with a porcine genomic Interferon alpha probe in order to confirm the mapping of this gene family on porcine chromosome 1.<\/jats:p>","DOI":"10.1002\/cyto.990070613","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T15:31:53Z","timestamp":1123515113000},"page":"582-588","source":"Crossref","is-referenced-by-count":17,"title":["Swine chromosomes: Flow sorting and spot blot hybridization"],"prefix":"10.1002","volume":"7","author":[{"given":"D.","family":"Grunwald","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Geffrotin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Chardon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Frelat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Vaiman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.24.7571"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.175.4019.273"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00364868"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990060302"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000132215"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.3.1147"},{"key":"e_1_2_1_8_2","first-page":"213","article-title":"Assignment of MHC in swine to chromosome 7 by in situ hybridization and serological typing","volume":"27","author":"Geffrotin C","year":"1984","journal-title":"Ann Genet (Paris)"},{"key":"e_1_2_1_9_2","first-page":"639","article-title":"Rabbit and pig gene mapping","volume":"40","author":"Gellin J","year":"1985","journal-title":"Cytogenet Cell Genet"},{"key":"e_1_2_1_10_2","unstructured":"GrunwaldD FrelatG RosenfeldC Le GoR:Variations of human flow karyotype according to the type of flow cytometer used. 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