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The lymphogranuloma venereum strain of <jats:italic>C. trachomatis<\/jats:italic> was grown in vitro in L cells, fixed in p\u2010formaldehyde, stained with fluorescein isothiocyanate (FITC)\u2010conjugated monoclonal antibody to the chlamydial major outer membrane protein, and analyzed flow cytometrically. Infected cells stained 50\u2013100 times more intensely than uninfected cells, and they could easily be discriminated by flow analysis. The number of infected cells and the fluorescence intensity of individual cells were proportional to the multiplicity of infection. The attachment of purified elementary bodies to L cells could be analyzed by immunofluorescence and flow cytometry. Cells exposed to 0.26 inclusion\u2010forming units\/cell could be discriminated from an unexposed population. Flow analysis of purified elementary bodies was possible after fluorescent staining with the aid of a laser\u2010based cytometer and gating on low volume.<\/jats:p>","DOI":"10.1002\/cyto.990080109","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T14:49:56Z","timestamp":1123512596000},"page":"55-59","source":"Crossref","is-referenced-by-count":10,"title":["Flow cytometric analysis of <i>Chlamydia trachomatis<\/i> interaction with L cells"],"prefix":"10.1002","volume":"8","author":[{"given":"Frederic M.","family":"Waldman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. Keith","family":"Hadley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mack J.","family":"Fulwyler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julius","family":"Schachter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"1060","article-title":"Influence of lectins, hexoses, and neuraminidase on the association of purified elementary bodies of Chlamydia trachomatis","volume":"40","author":"Bose SK","year":"1983","journal-title":"UW\u201031 with HeLa cells Infect Immun"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1128\/iai.48.1.78-82.1985","article-title":"Systemic Chlamydia trachomatis infection in mice: A comparison of lymphogranuloma venereum and trachoma biovars","volume":"48","author":"Brunham RC","year":"1985","journal-title":"Infect Immun"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1128\/iai.38.2.745-754.1982","article-title":"Neutralization of C. trachomatis infectivity with antibodies to MOMP","volume":"38","author":"Caldwell HD","year":"1982","journal-title":"Infect Immun"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.31.3.1161-1176.1981"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990060306"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1128\/iai.40.2.741-751.1983","article-title":"Cpsittaci elementary body envelopes: Ingestion and inhibition of phagolyso\u2010some fusion","volume":"40","author":"Eissenberg G","year":"1983","journal-title":"Infect Immun"},{"key":"e_1_2_1_8_2","first-page":"67","volume-title":"Instrumental Methods for Rapid Microbiological Analysis","author":"Hadley WK","year":"1985"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990030212"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1128\/aem.44.6.1258-1269.1982","article-title":"Application of flow cytometry to studies of pathogenic free\u2010living amoebae","volume":"44","author":"Muldrow LL","year":"1982","journal-title":"Appl Environ Microbiol"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.38.3.1181-1189.1982"},{"key":"e_1_2_1_12_2","first-page":"234","article-title":"Interaction of chlamydiae with host cells and mucous surfaces","volume":"80","author":"Pearce JH","year":"1981","journal-title":"Ciba Found Symp"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197802232980805"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.34.100180.001441"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.40.2.534-541.1983"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.42.3.930-935.1983"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1128\/jcm.17.4.666-668.1983","article-title":"Detection of Chlamydia trachomatis inclusions in McCoy cell cultures with fluoresceinconjugated monoclonal antibodies","volume":"17","author":"Stamm WE","year":"1983","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01651350"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990080109","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990080109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T07:32:59Z","timestamp":1697873579000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990080109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,1]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1987,1]]}},"alternative-id":["10.1002\/cyto.990080109"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990080109","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,1]]}}}