{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:50:51Z","timestamp":1761540651762},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":6276,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1988,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent reports, indicating that under some conditions the intensity of light scattering from cells is a nonlinear function of cell volume, have led to the widespread generalization that intensity of low\u2010angle light scattering indicates cell size. This study was performed to measure the relationships between light scattering and cell volumes in anisotonic solutions and after a freeze\u2010thaw stress.<\/jats:p><jats:p>Cell volumes in isolated human lymphocytes, human granulocytes, and hamster fibroblasts were deliberately altered by exposure to anisotonic solutions. Boyle\u2010vant Hoff plots of cell volume as a function of inverse osmotic pressure showed that the cells behaved as osmometers. Similar plots of right\u2010angle and low\u2010angle light scattering showed that the intensity of light scattering varied inversely with cell volume.<\/jats:p><jats:p>In other experiments where cells were frozen without cryoprotectant at various sub zero temperatures to \u221225\u00b0C and then thawed rapidly, cell viability decreased progressively with decreasing temperature, as did the intensity of both low\u2010angle and right\u2010angle light scattering, although cell volumes remained relatively constant.<\/jats:p><jats:p>The intensity of both low\u2010 and high\u2010angle light scattering varied inversely with cell volumes in hypertonic and hypotonic solutions, but cell damage induced by freezing and thawing resulted in significant reductions in the intensity of low\u2010angle light scattering with little change in cell volume.<\/jats:p><jats:p>These observations show that light scattering and cell volumes can vary independently, and they underline the need for a better understanding of the phenomenon of light scattering from living cells.<\/jats:p>","DOI":"10.1002\/cyto.990090106","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T15:36:24Z","timestamp":1123515384000},"page":"33-38","source":"Crossref","is-referenced-by-count":37,"title":["Light scattering and cell volumes in osmotically stressed and frozen\u2010thawed cells"],"prefix":"10.1002","volume":"9","author":[{"given":"Locksley E.","family":"McGann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michele L.","family":"Walterson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lori 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