{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T07:14:10Z","timestamp":1722842050660},"reference-count":10,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":6155,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1988,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Orthogonal and forward light\u2010scattering properties of lymphocytes were measured from patients with different lymphocytic diseases in order to determine the potential value of light scattering as a screening device. Monitoring of orthogonal light scattering of lymphocytes of a B\u2010cell chronic lymphocytic leukemia patient during splenic irradiation (SI) revealed the selective decrease of malignant cells and the fact that the major part of the residual lymphocytes were cytotoxic lymphocytes. By combining forward and orthogonal light scattering it was shown that lymphocytes from a patient with T<jats:sub>\u03bb<\/jats:sub> lymphocytosis were abnormal. Orthogonal light scattering also showed an increase in cytotoxic lymphocytes in a patient with mononucleosis infectiosa and in a splenectomized patient.<\/jats:p><jats:p>Orthogonal light scattering of lymphocyte subpopulations showed that the leu8<jats:sup>+<\/jats:sup> population of a patient with mononucleosis infectiosa was bidisperse. For elderly donors the occurrence of CD3<jats:sup>+<\/jats:sup>, CD4<jats:sup>+<\/jats:sup>, CD8<jats:sup>+<\/jats:sup>, and HNK\u20101<jats:sup>+<\/jats:sup> lymphocytes with a large orthogonal light scattering varied considerably. The CD8<jats:sup>+<\/jats:sup> lymphocytes of these donors consisted mainly of cytotoxic lymphocytes.<\/jats:p><jats:p>These results show that determination of light\u2010scattering properties of lymphocytes may yield important diagnostic information and can indicate when further investigation of the lymphocytes by means of immunofluorescence is necessary.<\/jats:p>","DOI":"10.1002\/cyto.990090306","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T15:26:06Z","timestamp":1123514766000},"page":"220-225","source":"Crossref","is-referenced-by-count":7,"title":["Application of orthogonal light scattering for routine screening of lymphocyte samples"],"prefix":"10.1002","volume":"9","author":[{"given":"L. W. M. M.","family":"Terstappen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. G.","family":"De Grooth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Greve","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"VanBerkel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. H. H.","family":"TenNapel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990050513"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(74)85925-4"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990080602"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(70)86334-2"},{"key":"e_1_2_1_6_2","first-page":"374","article-title":"Cell classification by laser light scattering: Identification and separation of unstained leukocytes","volume":"19","author":"Salzman GC","year":"1975","journal-title":"Acto Cytol (Baltimore)"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(86)90408-4"},{"key":"e_1_2_1_8_2","unstructured":"TerstappenLWMM de GroothBG van BerkelW ten NapelCHH GreveJ: Flow cytometric characterization of chronic lymphocytic leukemias using orthogonal light scattering and quantitative immunofluorescence.Blut(accepted for publication)."},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990070209"},{"key":"e_1_2_1_10_2","unstructured":"TerstappenLWMM de GroothBG van BerkelW ten NapelCHH GreveJ: Abnormal distribution of T8 subpopulation in B\u2010chronic lymphocytic leukemia identified by flow cytometry.Leuk Res(in press)."},{"key":"e_1_2_1_11_2","first-page":"391","article-title":"Light scattering properties of murene hematopoietic cells","volume":"6","author":"Visser JW","year":"1980","journal-title":"Blood Cells"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990090306","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990090306","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T18:01:38Z","timestamp":1697911298000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990090306"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,5]]},"references-count":10,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1988,5]]}},"alternative-id":["10.1002\/cyto.990090306"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990090306","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,5]]}}}