{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T21:53:16Z","timestamp":1698011596677},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":6094,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1988,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The leucoconcentration technique allows rapid obtainment of cellular suspensions from total blood or bone marrow for flow cytometric analysis. The technique is based on picric acid in ethyl alcohol fixation and saponin red cell lysis, followed by mithramycin staining for DNA. It gives a good resolution of DNA distributions that allow detection of slight variations in DNA content. These results were obtained with cellular suspensions differeing only in one X or Y chromosome (male, female, Klinefelter and Turner syndromes).<\/jats:p><jats:p>In these studies the ratio of the DNA content of X and Y chromosomes agrees with the chromosomal mass ratio already reported by other authors, but the \u201cabsolute values\u201d are 10\u2010fold more compared to these same works. Our conclusion is that leucoconcentration technique followed by DNA staining with mithramycin increases the difference in the dye's penetration and binding between X and Y chromosomes.<\/jats:p>","DOI":"10.1002\/cyto.990090405","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T16:09:05Z","timestamp":1123517345000},"page":"299-302","source":"Crossref","is-referenced-by-count":1,"title":["Flow cytometric analysis of DNA content differences in blood samples obtained by leucoconcentration"],"prefix":"10.1002","volume":"9","author":[{"given":"J.","family":"Pierrez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Guerci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Guerci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"577","article-title":"Flow fluorometric diagnosis of euploid and aneuploid human lymphocytes","volume":"28","author":"Callis J","year":"1976","journal-title":"Am J Hum Genet"},{"key":"e_1_2_1_3_2","first-page":"307","volume-title":"The Nucleic Acids I","author":"Chargaff E","year":"1955"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/28.10.6448270"},{"key":"e_1_2_1_5_2","first-page":"529","article-title":"Recherche des cellules canc\u00e9reuses dans le sang","volume":"6","author":"Herbeuval R","year":"1961","journal-title":"Rev Franc Geront"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000130801"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990040209"},{"key":"e_1_2_1_8_2","first-page":"1723","volume-title":"Pharmacologie Clinique","author":"Kenis Y","year":"1979"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.24.7876"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990050414"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-0973-4_16"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1986.tb02927.x"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990070402"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990080515"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000131553"},{"key":"e_1_2_1_16_2","first-page":"271","article-title":"High resolution ploidy determination by flow cytometric analysis with two internal standards","volume":"24","author":"Vindelov L","year":"1980","journal-title":"Basic Appl Histochem"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990030504"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990030505"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990090405","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990090405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T11:20:25Z","timestamp":1697887225000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990090405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,7]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1988,7]]}},"alternative-id":["10.1002\/cyto.990090405"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990090405","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,7]]}}}