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Also, we are able to show that the percentage of chromosomes which can be clearly distinguished by this procedure depends to a large extent on the duration of mitotic arrest.<\/jats:p><jats:p>In particular when sorting chromosomes from human\u2010rodent hybrid cell lines, the possibility of using in situ hybridization in addition to conventional staining techniques to characterize the chromosomes can help overcome the problem of highly condensed chromosomes and chromosomal fragments of unknown origin, which cannot be identified otherwise. Thus, we have developed an in situ hybridization technique, based on biotin\u2010labelled human genomic DNA, which allows a clear distinction between human and rodent chromosomal material to be made.<\/jats:p>","DOI":"10.1002\/cyto.990090517","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T15:54:13Z","timestamp":1123516453000},"page":"504-507","source":"Crossref","is-referenced-by-count":16,"title":["Identification of flow\u2010sorted chromosomes by G\u2010banding and in situ hybridization"],"prefix":"10.1002","volume":"9","author":[{"given":"B.","family":"Rommel","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.\u2010J.","family":"Hutter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Bullerdiek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Bartnitzke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Goerttler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Schloot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(86)90413-6"},{"key":"e_1_2_1_3_2","first-page":"75","article-title":"Effects of hypotonic treatment on human metaphase chromosome length","volume":"31","author":"Bartnitzke S","year":"1981","journal-title":"Cytobios"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(85)90075-5"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/24.1.1254929"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.22.8083"},{"key":"e_1_2_1_7_2","first-page":"159","volume-title":"Construction of human chromosome\u2010specific DNA libraries from flow\u2010sorted chromosomes","author":"Deaven LL","year":"1986"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01534722"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00389460"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02450482"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00286050"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00388453"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1159\/000132041"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.9.2934"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(71)90287-X"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00388452"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/27.1.86578"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00495052"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990090517","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990090517","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T04:33:34Z","timestamp":1697949214000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990090517"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,9]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1988,9]]}},"alternative-id":["10.1002\/cyto.990090517"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990090517","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,9]]}}}