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The cells\u2013differentiated into: (1) erythrocytes, (2) reticulocytes, (3) nucleated erythroid cells, (4) platelets, (5) lymphocytes, (6) monocytes, (7) neutrophils, (8) eosinophils, and (9) immature leukocytes\u2013had to meet unique criteria with regard to their characteristics in the created five\u2010dimensional space in order to be classified in a specific cell category.<\/jats:p><jats:p>Forward and orthogonal light\u2010scattering signals were matched with three fluorescence variables to obtain discrimination without necessitating erythrocyte lysis.<\/jats:p><jats:p>Thiazole orange (binding predominantly to RNA) and LDS\u2010751 (principally detecting DNA) were used to differentiate erythrocytes, platelets, reticulocytes, and nucleated cells. A monoclonal antibody, CD45, conjugated with phycoerythrin, was used to aid in discriminating between lineages of nucleated cells.<\/jats:p>","DOI":"10.1002\/cyto.990090607","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T14:18:24Z","timestamp":1123510704000},"page":"548-556","source":"Crossref","is-referenced-by-count":59,"title":["Five\u2010dimensional flow cytometry as a new approach for blood and bone marrow differentials"],"prefix":"10.1002","volume":"9","author":[{"given":"Leon W. M. 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