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Labeled chromosomes are examined on microscope slides and by flow cy tometry. In both cases a dye which binds to DNA is added to provide identification of the chromosome groups. Sera from different CREST patients vary in their ability to bind to ch romosome arms in addition to the centromeric region. Flow cytometry and microfluorimetry measurements have shown that with a given CREST serum the differences in kinetochore fluorescence between chromosomes are only minor. Flow cytometry experiments to relate the number of dicentric chromosomes, induced by in vitro radiation of peripheral blood cells to the slightly increased number of chromosomes with above\u2010average kinetochore fluorescence did not produce decisive radiation dosimetry results.<\/jats:p>","DOI":"10.1002\/cyto.990100204","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T15:36:02Z","timestamp":1123515362000},"page":"134-142","source":"Crossref","is-referenced-by-count":19,"title":["Flow cytometry measurements of human chromosome kinetochore labeling"],"prefix":"10.1002","volume":"10","author":[{"given":"Judith A.","family":"Fantes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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