{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T22:43:35Z","timestamp":1770504215333,"version":"3.49.0"},"reference-count":8,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":5729,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1989,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An automated image cytometry device, the Cell Analyzer, was used to locate live V79 cells plated at low densities in a tissue culture flask. Cells and other objects were detected by moving the flask in steps across a linear solid\u2010state image sensor. The step size was selected to be small enough to allow detection of all the cells in the area being scanned but sufficiently large so that most cells would be detected on only one image line. To distinguish cells from other detected objects, a recognition algorithm utilizing 18 characteristic cell signal features was developed. The algorithm first tests whether a set of feature values falls within specified upper and lower bounds, and then applies a linear discriminant function to the remaining data to further discriminate cells from debris. False\u2010positive errors of 5% or less were achieved with this method, whereas 15\u201335% of cells were misclassified as debris.<\/jats:p>","DOI":"10.1002\/cyto.990100403","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T15:42:11Z","timestamp":1123515731000},"page":"375-381","source":"Crossref","is-referenced-by-count":23,"title":["Automated detection and recognition of live cells in tissue culture using image cytometry"],"prefix":"10.1002","volume":"10","author":[{"given":"Ingrid","family":"Spadinger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven S. S.","family":"Poon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Branko","family":"Palcic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"980","article-title":"The design and development of an optical scanner for cell biology","volume":"2","author":"Jaggi B","year":"1985","journal-title":"IEEE Proc Eng Med Biol"},{"key":"e_1_2_1_3_2","first-page":"906","article-title":"Implementation and evaluation of the DMIPS Cell Analyzer","volume":"3","author":"Jaggi B","year":"1986","journal-title":"IEEE Proc Eng Med Biol"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553008614550721"},{"key":"e_1_2_1_5_2","volume-title":"Bioinstrumentation: Developments and Applications","author":"Palcic B","year":"1989"},{"key":"e_1_2_1_6_2","first-page":"1454","article-title":"Cell recognition algorithms for the Cell Analyzer","volume":"4","author":"Poon SSS","year":"1987","journal-title":"IEEE Proc Eng Med Biol"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.2307\/3571929"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.2307\/3572025"},{"key":"e_1_2_1_9_2","unstructured":"SpadingerI PalcicB: Effect of focus on cell detection and recognition by the Cell Analyzer (submitted)."}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990100403","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990100403","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T16:32:51Z","timestamp":1697905971000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990100403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,7]]},"references-count":8,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1989,7]]}},"alternative-id":["10.1002\/cyto.990100403"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990100403","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,7]]}}}