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In addition, probe pUC1.77 was used to mark the Iq12 subregion in normal and aberrant chromosomes 1. Low LET radiation (<jats:sup>60<\/jats:sup>Co\u2010\u03b3\u2010rays; 1.17 and 1.33 MeV) of lymphocyte cultures was performed with various doses (D = 0, 2, 4, 8 Gy) 5 h after stimulation with phytohaemagglutinin. Irradiated cells were cultivated for an additional 67 h before Colcemid arrested metaphase spreads were obtained. Aberrations of the specifically stained chromosomes, such as deletions, dicentrics, and rings, were readily scored after in situ hybridization with either the 1q12 specific probe or DNA\u2010library inserts. By the latter approach, translocations of the specifically stained chromosomes could also be reliably assessed. A linear increase of the percentage of specifically stained aberrant chromosomes was observed when plotted as a function of the square of the dose D. A particular advantage of this new approach is provided by the possibility to delineate numerical and structural chromosome aberrations directly in interphase nuclei. These results indicate that cytogenetic monitoring of ionizing radiation may be considerably facilitated by CISS\u2010hybridization.<\/jats:p>","DOI":"10.1002\/cyto.990110113","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T14:24:14Z","timestamp":1123511054000},"page":"110-118","source":"Crossref","is-referenced-by-count":100,"title":["Rapid metaphase and interphase detection of radiation\u2010induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization"],"prefix":"10.1002","volume":"11","author":[{"given":"T.","family":"Cremer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Popp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Emmerich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Lichter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Cremer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(87)90297-1"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.4048934"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-69334-2_2"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553008914550261"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01210837"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(73)90006-7"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/6.10.3177"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(86)90055-2"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990050604"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-74738-0_11"},{"key":"e_1_2_1_12_2","first-page":"9","article-title":"Flow cytometry of chromosomes: Principles and applications in medicine and molecular biology","volume":"82","author":"Cremer C.","year":"1989","journal-title":"Optik"},{"key":"e_1_2_1_13_2","first-page":"327","article-title":"Laser cytometry: Applications in flow cytogenetics","volume":"93","author":"Cremer C.","year":"1989","journal-title":"Phys Chem"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00280484"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01790091"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(88)90325-4"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(87)90256-9"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/293374a0"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00283626"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(89)90188-2"},{"key":"e_1_2_1_21_2","unstructured":"EmmerichP.:Interphasezytogenetische Untersuchungen an Gewebeschnitten und In Vitro Kultivierien Normalen Menschlichen Zellen Sowie an Hodenkeimzelltumoren. 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