{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T23:58:36Z","timestamp":1790899116073,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":5545,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1990,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ten carboxymethylindocyanine dyes which from the basis of a new series of fluorescent probes have been synthesized and converted into succinimidyl active esters for fluorescent labeling of proteins or other amino\u2010containing substances. Fluorescence emission maxima for members of the series range from 575 to 780 nm. Hydrophilic, water\u2010soluble reagents have been obtained which yield labeled antibodies with little tendency to form precipitates. The fluorescence intensities achieved are higher than those produced by labeling with the cyanine isothiocyanates described previosly (Mujumdar et al.: <jats:italic>Cytometry<\/jats:italic> 10:11\u201319, 1989). The utility of these reagents has been demonstrated in antibody labeling for two\u2010color immunofluorescent imaging of internal structures in a mammalian cell and for two\u2010color flow\u2010cytometry experiments. The use of values of chromophore\u2010equivalent weight (W\/Ceq), calculated from quantitative absorption data on dye samples, is proposed as an aid in formulating labeling procedures.<\/jats:p>","DOI":"10.1002\/cyto.990110313","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T16:27:59Z","timestamp":1123518479000},"page":"418-430","source":"Crossref","is-referenced-by-count":203,"title":["Cyanine dye labeling reagents\u2014carboxymethylindocyanine succinimidyl esters"],"prefix":"10.1002","volume":"11","author":[{"given":"Philip L.","family":"Southwick","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren A.","family":"Ernst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica W.","family":"Tauriello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen R.","family":"Parker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ratnakar B.","family":"Mujumdar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swati R.","family":"Mujumdar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hester A.","family":"Clever","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan S.","family":"Waggoner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)61757-X"},{"key":"e_1_2_1_3_2","volume-title":"Digital image processing","author":"Castleman KR","year":"1979"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.4.1613"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990100103"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.91.2.589"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780470186794"},{"key":"e_1_2_1_8_2","first-page":"29","volume-title":"Handbook of Fluorescent Probes and Research Chemicals","author":"Haughland RP","year":"1989"},{"key":"e_1_2_1_9_2","first-page":"219","volume-title":"Eastman Kodak Company","author":"Kodak Laboratory & Research Products","year":"1987"},{"key":"e_1_2_1_10_2","first-page":"50","volume-title":"Kodak Laser Dyes","year":"1987"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990100104"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/00304948809355822"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo00180a036"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(82)90225-3"},{"key":"e_1_2_1_16_2","first-page":"514","article-title":"Fluorescent localization of contractile proteins in tissue culture cells","volume":"185","author":"Wang K","year":"1984","journal-title":"Methods Enzymo"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990110313","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990110313","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T05:54:41Z","timestamp":1695707681000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990110313"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,1]]},"references-count":15,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,1]]}},"alternative-id":["10.1002\/cyto.990110313"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990110313","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,1]]}}}