{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T12:02:12Z","timestamp":1698580932854},"reference-count":12,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":5545,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1990,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Leukemia blasts isolated from bone marrow aspirates of 44 adults with acute leukemia were incubated for 1 h with 0.008\u201332 \u03bcM bromodeoxyuridine (Brd\u2010Urd). After dual labeling with monoclonal anti\u2010BrdUrd antibodies and propidium iodide, the cells were analyzed by flow cytometry. Percent labeled cells and intensity of labeling were similar over concentrations of BrdUrd ranging from 0.8\u201332 \u03bcM\u2014a 40\u2010fold range. Therefore, despite potential interpatient variability in nucleoside pharmacokinetics, commonly used doses of BrdUrd which are intended to achieve steady\u2010state plasma concentrations in the 8.0 \u03bcM range can be expected to provide a reliable estimate of the S\u2010phase fraction.<\/jats:p>","DOI":"10.1002\/cyto.990110315","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T16:27:59Z","timestamp":1123518479000},"page":"438-441","source":"Crossref","is-referenced-by-count":5,"title":["Bromodeoxyuridine incorporation into DNA of human leukemia cells is not concentration dependent"],"prefix":"10.1002","volume":"11","author":[{"given":"Bayard L.","family":"Powell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Betsy W.","family":"Gregory","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy E.","family":"Kute","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy M.","family":"Morgan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. Susan","family":"Lyerly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert L.","family":"Capizzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,21]]},"reference":[{"key":"e_1_2_1_1_2","first-page":"57","article-title":"Variations in ara\u2010C plasma concentrations at steady\u2010state (C\u2010ss) during remission induction and intensification therapy of AML. A population pharmacokinetics study by CALGB","volume":"7","author":"Capizzi RL","year":"1988","journal-title":"Proc Am Soc Clin Oncol"},{"key":"e_1_2_1_2_2","volume-title":"BMDP Statistical Software Manual","author":"Dixon WJ","year":"1988"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990060606"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.18.5573"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990060619"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0360-3016(84)90414-0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-8122-8"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1182\/blood.V69.6.1647.1647","article-title":"Differences in cell cycle characteristics among patients with acute nonlymphocytic leukemia","volume":"69","author":"Raza A","year":"1987","journal-title":"Blood"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990060620"},{"key":"e_1_2_1_10_2","first-page":"1702","article-title":"Pharmacological evaluation of intravenous delivery of 5\u2010bromodeoxyuridine to patients with brain tumors","volume":"44","author":"Russo A","year":"1984","journal-title":"Cancer Res"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990070602"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1988.234"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990110315","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990110315","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T05:54:45Z","timestamp":1695707685000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990110315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,1]]},"references-count":12,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,1]]}},"alternative-id":["10.1002\/cyto.990110315"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990110315","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,1]]}}}