{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,30]],"date-time":"2025-09-30T04:40:03Z","timestamp":1759207203432},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":5545,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1990,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous studies on cultured cells revealed that the values of certain shape descriptors were highly correlated despite the fact that there was little mathematical interdependence (Heckman, CA: In Advances in Cell Culture, Vol. 4, K. Maramorosch, ed, pp 85\u2013156, 1985). The independence of such descriptors was tested by calculating the values of correlations among descriptors for a data set consisting of macroscopic objects. The descriptors were unlinked for this data set, confirming that high correlations reflected specific characteristics of cultured cells. These characteristics were identified by constructing model figures incorporating the geometrical features postulated to be responsible for each correlation. Because the position of the ellipse of concentration depended upon the form in which mass was displaced in figures, the fraction of the figure falling within the area of the ellipse (FINE) and the ratio of the ellipse area to the figure's area (ARAT) became decorrelated from each other in figures with massive projections. Further, correlations between ARAT or FINE and variables measuring cavities and inflections of the perimeter showed a complex dependence on the size, shape, and number of invaginations. Descriptors could become decorrelated due to features present in only a minority of cells of a population. Finally, cell populations that differed with respect to physiological characteristics showed no differences in the value of ARAT:FINE correlation. However, correlations between ARAT and the coefficient of variation of centroid\u2010to\u2010perimeter distances were altered. This suggested that correlation coefficients may be themselves powerful descriptors of the shape characteristics of cell populations.<\/jats:p>","DOI":"10.1002\/cyto.990110703","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T16:05:20Z","timestamp":1123517120000},"page":"771-783","source":"Crossref","is-referenced-by-count":10,"title":["Geometrical constraints on the shape of cultured cells"],"prefix":"10.1002","volume":"11","author":[{"given":"C. A.","family":"Heckman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1242\/jcs.54.1.47","article-title":"Cell shape and organization of cytoskeleton and surface fibronectin in non\u2010tumorigenic and tumorigenic rat liver cultures","volume":"54","author":"Bannikov GA","year":"1982","journal-title":"J Cell Science"},{"key":"e_1_2_1_3_2","first-page":"329","article-title":"Statistical analysis of morphometrically analyzed outlines of cells in culture: Comparison of populations of normal and polyomavirus\u2010transformed FR 3T3 fibroblasts","volume":"10","author":"Cibert C","year":"1988","journal-title":"Anal Quant Cytol Histol"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990100107"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.12.4122"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(79)90011-9"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.10.4466"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02616178"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-007904-9.50010-1"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(87)90232-1"},{"key":"e_1_2_1_11_2","first-page":"2390","article-title":"Morphological markers of oncogenic transformation in respiratory tract epithelial cells","volume":"39","author":"Heckman CA","year":"1979","journal-title":"Cancer Res"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(77)90024-6"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1177\/26.11.569164"},{"key":"e_1_2_1_14_2","first-page":"307","volume-title":"Mecanisms d'Alt\u00e9ration et de R\u00e9paration du DNA: Relations avec la Mutagen\u00e8se et la Carcerogen\u00e8se Chimique","author":"Kuroki T","year":"1977"},{"key":"e_1_2_1_15_2","first-page":"4591","article-title":"Structural anomalies of highly malignant respiratory tract epithelial cells","volume":"42","author":"Manger RL","year":"1982","journal-title":"Cancer Res"},{"key":"e_1_2_1_16_2","first-page":"2030","article-title":"In vitro development of oncogenicity in cell lines established from tracheal epithelium pre\u2010exposed in vivo to 7,12\u2010dimethylbenz(a)anthracene","volume":"38","author":"Marchok AC","year":"1978","journal-title":"Cancer Res"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.3.1516"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(90)90279-J"},{"key":"e_1_2_1_19_2","first-page":"315","article-title":"Morphometric analysis of the cell outlines of normal and polyomavirus\u2010transformed FR 3T3 fibroblasts","volume":"9","author":"Schoevaert D","year":"1987","journal-title":"Anal Quant Cytol Histol"},{"key":"e_1_2_1_20_2","first-page":"207","article-title":"Metabolism of ether\u2010linked glycerolipids in cultures of normal and neoplastic rat respiratory tract epithelium","volume":"39","author":"Scott CC","year":"1979","journal-title":"Cancer Res"},{"key":"e_1_2_1_21_2","first-page":"3563","article-title":"Establishment of epithelial cell lines following exposure of cultured tracheal epithelium to 12\u20100\u2010tetradecanoyl\u2010phorbol\u201013\u2010acetate","volume":"38","author":"Steele VE","year":"1978","journal-title":"Cancer Res"},{"key":"e_1_2_1_22_2","first-page":"68","article-title":"Cytoplasmic and nuclear shape analysis for computerized cell recognition","volume":"20","author":"Sychra JJ","year":"1976","journal-title":"Acta Cytologica"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.11.4065"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(77)90138-6"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990110703","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990110703","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T23:14:00Z","timestamp":1698016440000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990110703"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,1]]},"references-count":23,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1990,1]]}},"alternative-id":["10.1002\/cyto.990110703"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990110703","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,1]]}}}