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Chromosomes were then isolated, stained with propidium iodide, and analyzed using SSFCM for total fluorescence and slit\u2010scan profile. The frequency of chromosomes having DNA contents greater than once and less than twice the DNA content of the number 1 chromosome and producing trimodal profiles was determined at each dose. This frequency was used as an estimate of the relative dicentric chromosome frequency at that dose. The estimated dicentric chromosome frequency per cell, f(D), increased with dose, D, in a linear\u2010quadratic manner according to the relation <jats:disp-formula>\n\n<\/jats:disp-formula><\/jats:p>","DOI":"10.1002\/cyto.990120405","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T15:04:08Z","timestamp":1123513448000},"page":"316-322","source":"Crossref","is-referenced-by-count":9,"title":["Dicentric chromosome frequency analysis using slit\u2010scan flow cytometry"],"prefix":"10.1002","volume":"12","author":[{"given":"Joe N.","family":"Lucas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James C.","family":"Mullikin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joe W.","family":"Gray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,21]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"287","volume-title":"Flow Cytometry","author":"Aten J","year":"1980"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.2307\/3572080"},{"key":"e_1_2_1_4_2","first-page":"326","volume-title":"Mutagen\u2010Induced Chromosome Damage in Man","author":"Carrano AV","year":"1978"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(85)90061-2"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/277531a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990100204"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bb.15.060186.001211"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-69334-2_3"},{"key":"e_1_2_1_10_2","first-page":"375","volume-title":"The Medical Basis for Radiation Accident Preparedness","author":"Littlefield G","year":"1980"},{"key":"e_1_2_1_11_2","first-page":"215","article-title":"Doses in radiation accidents investigated by chromosome aberration analysis. 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A review of cases investigated","volume":"128","author":"Lloyd D","year":"1981","journal-title":"UK Natl Radiol Protect Bd RepR."},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553007514550781"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(80)90123-2"},{"key":"e_1_2_1_14_2","first-page":"173","volume-title":"Flow Cytometry and Cell Sorting","author":"Lucas JN","year":"1989"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990070612"},{"key":"e_1_2_1_16_2","first-page":"B4\u201021","volume-title":"Radiation Research, Somatic and Genetic Effects","author":"Lucas JN","year":"1983"},{"key":"e_1_2_1_17_2","first-page":"13","article-title":"Automatic quantification of the frequency of aberrant chromosome in human cells","volume":"1","author":"Lucas JN","year":"1987","journal-title":"Cytometry Suppl"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553008914551161"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/2201189a0"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990120405","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990120405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:28:53Z","timestamp":1698020933000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990120405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,1]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1991,1]]}},"alternative-id":["10.1002\/cyto.990120405"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990120405","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,1]]}}}