{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T09:10:29Z","timestamp":1698225029763},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":4815,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The purpose of this study was to investigate the influence of different cell extraction procedures on relative nuclear DNA content (IOD), nuclear area, and texture features of Feulgen\u2010stained nuclei. In imprints and smears of fineneedle aspirates and suspensions from one human liver specimen, 50 diploid, 50 tetraploid, and 25 octaploid nuclei were measured from each slide. In addition, for DNA measurements, the progressive mean of IOD and tetraploid\/diploid and octaploid\/diploid ratios was calculated. The results show that the progressive mean of the IOD is constant after measuring 25\u201330 nuclei. For the three type of specimens, the IOD of diploid nuclei varied slightly. The average coefficient of variation was about 5% for the fineneedle aspirates, imprints, and suspensions. For all tissue sampling methods, the 99% confidence limits of the tetraploid\/diploid ratio and octaploid\/diploid ratio were within the range of 1.9\u20132.1 and 3.7\u20134.3, respectively. The nuclear area and most of the texture features showed a significant difference (p &lt; 0.01) between the three sampling methods in all nuclear populations. In conclusion, different tissue sampling methods have little or no effect on DNA\u2010related IOD measurements, whereas the outcome of nuclear area and texture features is very dependent of the cell extraction procedure.<\/jats:p>","DOI":"10.1002\/cyto.990130506","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T16:08:11Z","timestamp":1123517291000},"page":"485-489","source":"Crossref","is-referenced-by-count":2,"title":["Influence of different cell extraction methods on cytometric features"],"prefix":"10.1002","volume":"13","author":[{"given":"F. B. J. 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