{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:57:13Z","timestamp":1784249833131,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":4449,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CD34<jats:sup>+<\/jats:sup> cells, present at a frequency of 0.18 \u00b1 0.052% among leukocytes from peripheral blood (PB), can be rapidly and efficiently (recovery of 39.0\u201374.0%) enriched to a frequency of 38.6\u201387.1% (54.4 \u00b1 12.3%) by high\u2010gradient magnetic cell separation (MACS) for immunophenotyping, characterization in colony\u2010forming cell (CFU) assays, and further purification to homogeneity (&gt;98%) by multiparameter fluorescence\u2010activated cell sorting (FACS). Enriching PB\u2010CD34<jats:sup>+<\/jats:sup> cells for immunophenotyping allows the detection of small subpopulations, expressing the B\u2010cell antigens CD10, CD19, and CD20, the T\u2010cell antigens CD45RA and CD7, and a small subpopulation expressing high levels of CD34 (1.20 \u00b1 0.12%), which mostly coexpress CD19 (91.9 \u00b1 9.05%), CD20 (64.8 \u00b1 14.4%), and CD38 (84.5 \u00b1 10.3%). All PB\u2010CD34<jats:sup>+<\/jats:sup> cells express elevated levels of CD71 (transferrin receptor), with a subpopulation of high expressing cells, and CD38. Some cells express CD33. MACS\u2010enriched PBCD34<jats:sup>+<\/jats:sup> cells show \u201cnormal\u201d hematopoietic colony formation in vitro. The ease and efficiency of purification of large numbers of CD34<jats:sup>+<\/jats:sup> cells from PB by MACS is not only relevant for the characterization of migrating stem cells but also opens new possibilities for stem cell transplantation and genetic manipulation of the hematopoietic system. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.990140407","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T14:31:32Z","timestamp":1123511492000},"page":"384-392","source":"Crossref","is-referenced-by-count":105,"title":["Isolation and characterization of CD34<sup>+<\/sup> hematopoietic stem cells from human peripheral blood by high\u2010gradient magnetic cell 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