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The nonrandom distribution of the centromeres and their dynamic rearrangement during the cell cycle have been well documented. A close proximity of specific centromeres to nucleoli has also been reported, but the functional meaning of this association is still unknown. In order to investigate whether the chromosome 1 centromere region to nucleolus association depends on the cell cycle and chromosome status, we combined FISH of probes specific for the 1q12 region with Ki\u201067 nucleolar antigen fluorescent immunocytochemical (FICC) detection on the MCF\u20107 human breast cancer cell line and on the MRC\u20105 normal fibroblastic cell line. Both FISH and FICC signals were interactively localized in a one\u2010step fluorescent microscopic observation and further analyzed using the Highly Optimized Microscope Environment (HOME) graphics microscope workstation, which provided computerized interactive marking of 1q12 to nucleolus associations (1q12\u2010nu) at the individual nucleus and nucleolus levels. This study confirms that centromeric regions, other than those adjacent to the major ribosomal cistrons, contribute to the perinucleolar chromatin and demonstrate that, during the cell cycle, the heterochromatic band 1q12 is dynamically rearranged with regard to both the nuclear volume and the nucleoli. A relationship between the association of the chromosome 1 pericentromeric region with nucleoli and the nucleolar transcriptional activity is also strongly suggested. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.990160405","type":"journal-article","created":{"date-parts":[[2005,6,30]],"date-time":"2005-06-30T21:22:28Z","timestamp":1120166548000},"page":"313-323","source":"Crossref","is-referenced-by-count":34,"title":["Interactive computer\u2010assisted analysis of chromosome 1 colocalization with nucleoli"],"prefix":"10.1002","volume":"16","author":[{"given":"Isabelle","family":"L\u00e9ger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martial","family":"Guillaud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Krief","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00e9rard","family":"Brugal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1242\/jcs.99.2.255","article-title":"Nuclear distribution of centromeres during the cell cycle of human diploid fibroblasts","volume":"99","author":"Bartholdi MF","year":"1991","journal-title":"J Cell Sci"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3201257"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.91.1.95"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990130202"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00272388"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/6.10.3177"},{"key":"e_1_2_1_8_2","first-page":"5825","article-title":"Detection of chromosome aneuploidy in interphase nuclei from primary breast tumors using chromosome\u2010specific repetitive DNA probes","volume":"48","author":"Devilee P","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(89)90188-2"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00660315"},{"key":"e_1_2_1_11_2","first-page":"177","article-title":"Cell nucleus pattern analysis: Geometric and densito\u2010metric featuring, automatic cell phase identification","volume":"44","author":"Giroud F","year":"1982","journal-title":"Bio Cell"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(68)90422-9"},{"key":"e_1_2_1_13_2","first-page":"25","article-title":"Quantification and topographical description of Ki\u201067 antibody labelling during the cell cycle of normal fibroblastic (MRC\u20105) and mammary tumor (MCF\u20107) cell lines","volume":"1","author":"Guillaud P","year":"1989","journal-title":"Anal Cell Pathol"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00293889"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(90)90051-B"},{"key":"e_1_2_1_16_2","first-page":"192","volume-title":"The Nucleolus and Ribosome Biogenesis","author":"Hadjiolov AA","year":"1986"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(86)90199-0"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1242\/jcs.98.1.99","article-title":"Behaviour of nucleolar proteins in nuclei lacking ribosomal genes. 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