{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:25:29Z","timestamp":1789194329138,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":3841,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1994,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The rhesus monkey (<jats:italic>Macaca mulatta<\/jats:italic>) is an important experimental animal frequently utilized for studies of infectious diseases, immunity, hematopoiesis, and transplantation. Since the structure of cell surface molecules is phylogenetically conserved, monoclonal antibodies raised against human leukocyte antigens can sometimes recognize the homologous determinant on monkey leukocytes. To facilitate better utilization of this animal model, we tested 89 commercially available monoclonal antibodies which define 27 human cell surface antigens for reactivity with rhesus monkey PBL. Certain antigens which delineate clinical useful lymphocyte subsets such as CD2, CD4, CD8, CD14, CD16, CD20, and MHC class II are apparently well conserved since most human cell\u2010specific antibodies identified the homologous cell subset in monkeys. However, other antigens such as CD3, CD19, CD45, and CD56 were identified infrequently by human cell\u2010specific antibodies. FITC\u2010modification of antibodies which had no effect on their binding to human cells occasionally inhibited antibody binding to monkey cells. Never\u2010theless, an adequate number of cross\u2010reactive monoclonal antibodies was identified to allow gating of lymphocytes for accurate flow cytometric analysis and quantitation of the major lymphocyte subsets of the rhesus monkey. The T lymphocyte subset distribution in blood and lymphoid tissue of rhesus monkeys was similar to man. However, the B subset was significantly larger in monkeys. The daily variation in absolute PBL subset size was marked and found to be due mainly to daily fluctuations in total lymphocyte number. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.990170113","type":"journal-article","created":{"date-parts":[[2005,6,30]],"date-time":"2005-06-30T20:20:24Z","timestamp":1120162824000},"page":"102-108","source":"Crossref","is-referenced-by-count":80,"title":["Use of human leukocyte\u2010specific monoclonal antibodies for clinically immunophenotyping lymphocytes of rhesus monkeys"],"prefix":"10.1002","volume":"17","author":[{"given":"Keith A.","family":"Reimann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry C. D.","family":"Waite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David E.","family":"Lee\u2010Parritz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyu","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Uchanska\u2010Ziegler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"O'Connell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman L.","family":"Letvin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajp.1350170202"},{"key":"e_1_2_1_3_2","first-page":"190","article-title":"Effects of capture and venipuncture on serum levels of prolactin, growth hormone and cortisol in outdoor compound\u2010housed female rhesus monkeys (Macaca mulatta )","volume":"102","author":"Blank MS","year":"1983","journal-title":"Acta Endocrinol"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.4049\/jimmunol.134.4.2281","article-title":"Coexpression of T4 and T8 on peripheral blood T cells demonstrated by two\u2010color fluorescence flow cytometry","volume":"134","author":"Blue M\u2010L","year":"1985","journal-title":"J Immunol"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990140703"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00345968"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/321872a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(86)90183-8"},{"key":"e_1_2_1_9_2","first-page":"174","volume-title":"Manual of Clinical Laboratory Immunology","author":"Giorgi JV","year":"1992"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0889-1591(92)90018-J"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6171885"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"2117","DOI":"10.4049\/jimmunol.126.6.2117","article-title":"A human T lymphocyte differentiation marker defined by monoclonal antibodies that block E\u2010rosette formation","volume":"126","author":"Howard FD","year":"1981","journal-title":"J Immunol"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990140212"},{"key":"e_1_2_1_14_2","first-page":"1058","volume-title":"Leucocyte Typing IV","author":"Jonker M","year":"1989"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3287624"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830060411"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830130414"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(90)90127-U"},{"key":"e_1_2_1_19_2","first-page":"164","volume-title":"Manual of Clinical Laboratory Immunology","author":"Lewis DE","year":"1992"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990110402"},{"key":"e_1_2_1_21_2","first-page":"144","article-title":"Sources of variability in repeated T\u2010helper lymphocyte counts from human immunodeficiency virus type 1\u2010infected patients: Total lymphocyte count fluctuations and diurnal cycle are important","volume":"3","author":"Malone JL","year":"1990","journal-title":"J Acquir Immune Defic Synd"},{"key":"e_1_2_1_22_2","volume-title":"Quality assurance and immunophenotyping of peripheral blood lymphocyte; Tentative Guideline. NCCLS Document H42\u2010T (IBSN 1\u201356238\u2013155\u20135)","year":"1992"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830160817"},{"key":"e_1_2_1_24_2","first-page":"3","article-title":"Toward better health. The role of primates in biomedical research at the regional primate research centers","volume":"24","year":"1990","journal-title":"Primate News"},{"key":"e_1_2_1_25_2","volume-title":"Animal Models in AIDS","author":"Schellekens H","year":"1990"},{"key":"e_1_2_1_26_2","unstructured":"SchwinzerR: Cluster report: CD45\/CD45R In:Leucocyte Typing IV. Knapp W Dorken B Gilks WR Rieber EP Schmidt RE Stein H von dem Borne AEGKr (eds). 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