{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T05:44:20Z","timestamp":1698212660648},"reference-count":11,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":3811,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Utilizing flow cytometry, we previously demonstrated that the potassium channel blocker margatoxin (MgTX) inhibits the[Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> transient involved in T\u2010cell activation. We wished to extend these studies to single\u2010cell transients using florescence digital\u2010imaging microscopy (DIM). However, the most currently available temperature\u2010regulation chambers reuse part or all of the apparatus and introduce compounds via perfusion. Thus, these apparatuses are not suitable for studies involving compounds that are particularly sticky. We have designed a dual\u2010temperature regulation system that will maintain Nunc, eight\u2010well, coverglass\u2010bottom, disposable chambers, and three disposable addition pipets at 37\u00b0C for physiological studies on an inverted digital\u2010imaging microscope. We have demonstrated that calcium transients of human T lymphocytes can be initiated and monitored reproducibly during the addition of three distinct chemical species. The DIM results correlate with flow cytometry measurements in the number of responding cells and the heterogeneity of the response in both control and MgTX\u2010inhibited cultures. Additionally, DIM revealed that the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> transient is more rapid than the flow\u2010cytometric measurement indicated. The correlation between flow cytometry and DIM permits the interpretation of studies on the regulation of T\u2010cell activation. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.990170204","type":"journal-article","created":{"date-parts":[[2005,6,30]],"date-time":"2005-06-30T20:20:35Z","timestamp":1120162835000},"page":"128-134","source":"Crossref","is-referenced-by-count":4,"title":["A disposable\u2010chamber temperature\u2010regulation system for the study of intracellular calcium levels in single live T cells using fluorescence digital\u2010imaging microscopy"],"prefix":"10.1002","volume":"17","author":[{"given":"R. C. \u201cDutch\u201d","family":"Boltz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Sirotina","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom","family":"Blake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary","family":"Kath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian","family":"Uhrig","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"McKeel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Colleen","family":"Quinn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/cm.970070108"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-130-11-2915"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.104.4.1019"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(71)90596-9"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02618064"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"18866","DOI":"10.1016\/S0021-9258(17)46707-X","article-title":"Purification, characterization, and biosynthesis of margatoxin, a component of Centruoides margaritatus venom that selectively inhibits voltage\u2010dependant potassium channels","volume":"268","author":"Garcia\u2010Calvo M","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00583594"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90354-X"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.3.637"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(91)90103-I"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1993.1077"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990170204","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990170204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T05:00:27Z","timestamp":1698123627000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990170204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10]]},"references-count":11,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,10]]}},"alternative-id":["10.1002\/cyto.990170204"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990170204","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10]]}}}