{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:29:50Z","timestamp":1759336190500},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":3919,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1994,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tissue typing can help in the diagnosis of the seronegative arthropathy ankylosing spondylitis. Using an automatic sample preparation system and flow cytometry (FACSPrep\/FACScan) we have developed a test for HLA\u2010B27 screening using whole blood which is both rapid, reproducible, and permits simultaneous screening of large numbers of samples. We have used both indirect (248 cases) and direct (126 cases) monoclonal antibody staining techniques. Results were assessed using median channel shift (CS) from the negative control and relative fluorescence intensity. There is known cross\u2010reactivity between HLA\u2010B27 and HLA\u2010B7 with the monoclonal antibody (MoAb) HLA\u2010ABC\u2010m3. Using this antibody and indirect staining, HLA\u2010B7 samples had a significantly lower CS value than HLA\u2010B27 samples. In 60% of these cases there was a clear distinction between HLA\u2010B27 and HLA\u2010B7. All HLA\u2010B27 and HLA\u2010B7 negative samples had a CS of 0 (P &lt; 0.001). Using direct dual staining with CD3 and HLA\u2010B27, all HLA\u2010B7 negative samples had a low CS value (15 maximum), HLA\u2010B7 samples had an intermediate CS value (20\u201385), and HLA\u2010B27 samples had the highest CS values (70 upward). This system permits large scale rapid negative screening of samples with the elimination of over 60% as negatives (CS &lt; 15). Limitations as a definitive test for HLA\u2010B27 are due to MoAb cross\u2010reactivity with HLA\u2010B7 which necessitates other confirmatory techniques. The substitution of the HLA\u2010ABC\u2010m3 with the recently available HLA\u2010B27 specific MoAb FD705 would substantially increase the value of this technique for routine HLA\u2010B27 typing. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.990180210","type":"journal-article","created":{"date-parts":[[2005,6,30]],"date-time":"2005-06-30T20:48:51Z","timestamp":1120164531000},"page":"109-115","source":"Crossref","is-referenced-by-count":10,"title":["Automated HLA\u2010B27 testing using the FACSPrep\/FACScan system"],"prefix":"10.1002","volume":"18","author":[{"given":"Wendy M.","family":"Reynolds","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip R.","family":"Evans","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew C.","family":"Lane","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. Martin","family":"Howell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond","family":"Wong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John L.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"1619","DOI":"10.1093\/clinchem\/33.9.1619","article-title":"HLA\u2010B27 typing by use of flow cytofluorometry","volume":"33","author":"Albrecht J","year":"1987","journal-title":"Clin Chem"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-3552-1_37"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90296-9"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(73)91360-3"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-92-2-208"},{"issue":"1","key":"e_1_2_1_7_2","first-page":"289","article-title":"sHLA\u2010STATTMB27, an ELISA for HLA typing using serum","volume":"32","author":"Chang CH","year":"1991","journal-title":"Human Immunol"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.51.10.1138"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-69770-8_65"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.3109\/03009749009097079"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-69770-8_91"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)92452-8"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-313X.1991.tb00008.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1177\/000456329202900608"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00274931"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(89)90261-2"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.51.4.456"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.51.8.929"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-313X.1991.tb00032.x"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1993.tb02003.x"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0198-8859(92)90281-Q"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/69.2.103"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-8545-5"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0198-8859(83)90058-7"},{"key":"e_1_2_1_25_2","first-page":"311","article-title":"Expression of HLA\u2010B27 antigens on mononuclear leucocytes in ankylosing spondylitis","volume":"60","author":"van der Gaag R","year":"1985","journal-title":"Clin Exp Immunol"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990180210","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990180210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T17:35:48Z","timestamp":1698168948000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990180210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,6,15]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,6,15]]}},"alternative-id":["10.1002\/cyto.990180210"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990180210","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,6,15]]}}}