{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:17:58Z","timestamp":1790518678030,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":3538,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1995,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In an effort to improve detection of P\u2010glycoprotein\u2010mediated multidrug resistance (mdr), several dyes were compared to rhodamine 123 (R123) in efflux assays. Two dyes (SY\u201038 and SY\u20103150) were found that provided better sensitivity. These dyes were effluxed by a cell line known to be mdr\u2010positive (P388\/R84) but not by an mdr\u2010negative cell line (P388). Efflux was blocked by both verapamil and cyclosporine A. Efflux from KG1a cells was less than from P388\/R84, just as has been seen with R123 and daunomycin. In further experiments, a model system was used to demonstrate two\u2010color immunofluorescence plus efflux measurements. This was done using a sequential staining method. A procedure was devised that, at least for this model system, allowed single\u2010step staining with both dye and antibody. The sensitivity of the efflux measurement was slightly compromised by using this one\u2010step staining method. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.990200305","type":"journal-article","created":{"date-parts":[[2005,6,30]],"date-time":"2005-06-30T18:50:31Z","timestamp":1120157431000},"page":"218-227","source":"Crossref","is-referenced-by-count":19,"title":["Dyes providing increased sensitivity in flow\u2010cytometric dye\u2010efflux assays for multidrug resistance"],"prefix":"10.1002","volume":"20","author":[{"given":"Tom","family":"Frey","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Yue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard P.","family":"Haugland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199112053252304"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90141-K"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0198-8859(91)90110-U"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990120708"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/84.9.711"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)80566-3"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.3.14.2574119"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/316820a0"},{"key":"e_1_2_1_10_2","first-page":"1300","article-title":"Surface IgM\u2010kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines","volume":"28","author":"Klein E","year":"1968","journal-title":"Cancer Res"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V56.2.265.265"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990080618"},{"key":"e_1_2_1_13_2","first-page":"3895","article-title":"Laser flow cytometric studies on the intracellular fluorescence of anthracyclines","volume":"40","author":"Krishan A","year":"1980","journal-title":"Cancer Res"},{"key":"e_1_2_1_14_2","first-page":"3658","article-title":"The MRP gene associated with a non\u2010P\u2010glycoprotein multidrug resistance encodes a 190\u2010kDa membrane bound glycoprotein","volume":"53","author":"Krishnamachary N","year":"1993","journal-title":"Cancer Res"},{"key":"e_1_2_1_15_2","unstructured":"LeeLG ChenC\u2010H: Detection of reticulocytes DNA and RNA. US Patent Number 4 957 870 1987."},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1992.tb04608.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910210207"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(90)90151-A"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(88)90152-8"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00685031"},{"key":"e_1_2_1_21_2","first-page":"963","article-title":"High expression of the multidrug resistance P\u2010glycoprotein in high\u2010risk myelodysplasia is associated with immature phenotype","volume":"7","author":"Sonneveld P","year":"1993","journal-title":"Leukemia"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(05)80216-3"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990200305","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990200305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T03:10:34Z","timestamp":1698289834000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990200305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,7]]},"references-count":21,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,7]]}},"alternative-id":["10.1002\/cyto.990200305"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990200305","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,7]]}}}