{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T23:21:40Z","timestamp":1700176900038},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":3462,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1995,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The clinical, biological, and immunophenotypical characteristics of B\u2010cell chronic lymphocytic leukemia (B\u2010CLL) patients with trisomy 12 detected by fluorescence in situ hybridization (FISH) using a chromosome 12 alpha\u2010centromeric probe (D12Z3) were analyzed in the present study. From a total of 104 consecutive B\u2010CLL patients, 21 (20%) displayed trisomy 12, the percentage of trisomic cells ranging from 13% to 76%. From the clinico\u2010biological point of view, patients with trisomy 12 were associated with atypical CLL morphology (43% vs. 10%, <jats:italic>P<\/jats:italic> = 0.04) and BM diffuse pattern (75% vs. 25%, <jats:italic>P<\/jats:italic> = 0.02) together with increased WBC counts (141 \u00b1 220 vs. 58 \u00b1 67 \u00d7 10<jats:sup>9<\/jats:sup>\/L, <jats:italic>P<\/jats:italic> = 0.04). In contrast, no association was detected between the presence of trisomy 12 and other disease characteristics such as age, sex, clinical stage, hepatomegaly, lymphadenopathies, haemoglobin levels and platelet counts, and the cell cycle distribution of PB leukocytes in both groups of patients.<\/jats:p><jats:p>Trisomy 12 patients had a significantly higher expression of the FMC7 antigen both in percentage (34 \u00b1 34% vs. 13 \u00b1 20%, <jats:italic>P<\/jats:italic> = 0.02) and absolute numbers (29 \u00b1 62 vs. 7 \u00b1 17 \u00d7 10<jats:sup>9<\/jats:sup>\/L, <jats:italic>P<\/jats:italic> = 0.007). No major differences were found regarding the expression of mouse rosettes, CD19+, and CD19+ \/CD5 + lymphocytes.<\/jats:p><jats:p>Upon analyzing the correlations between the disease characteristics of trisomy 12 cases, significant associations were found between the percentage of trisomic cells and both the WBC count (r = 0.52, <jats:italic>P<\/jats:italic> = 0.02) and the PB lymphocyte count (r = 0.60, <jats:italic>P<\/jats:italic> = 0.007).<\/jats:p><jats:p>In summary, our results show that although trisomy 12 in B\u2010CLL is not associated with the clinical stage, it correlates with several prognostic factors and other disease characteristics such as an atypical lymphocyte morphology BM diffuse pattern, high WBC counts, and FMC7 expression. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.990220309","type":"journal-article","created":{"date-parts":[[2005,6,30]],"date-time":"2005-06-30T21:48:03Z","timestamp":1120168083000},"page":"217-222","source":"Crossref","is-referenced-by-count":19,"title":["Clinical, biological, and immunophenotypical characteristics of B\u2010cell chronic lymphocytic leukemia with trisomy 12 by fluorescence in situ hybridization"],"prefix":"10.1002","volume":"22","author":[{"given":"Maria Dolores","family":"Tabernero","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesus F.","family":"San Miguel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Luis","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Garcia\u2010Isidoro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joop","family":"Wiegant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juana","family":"Ciudad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos","family":"Gonzalez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agustin","family":"Rios","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anton","family":"Raap","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Orfao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0145-2126(83)90052-8"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830310105"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(88)90180-8"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1986.tb07575.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1987.tb06130.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1182\/blood.V64.3.642.642","article-title":"Bone marrow histologic pattern. 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