{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:31:43Z","timestamp":1791102703250,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,3,19]],"date-time":"2003-03-19T00:00:00Z","timestamp":1048032000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>A relation between the changes in DNA content and chromatin supra\u2010organization and the expression of gradual steps of tumorigenesis has been assessed by image analysis in human breast epithelial cells (MCF\u201010F) treated with benzo[a]pyrene (BP) (cell lines BP1, BP1\u2010E, BP1\u2010T<jats:italic>ras<\/jats:italic>, and others).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Because abnormal chromosomes 11 and 17 have been associated with neoplastic progression in BP\u2010transformed MCF\u201010F cells, image analysis of Feulgen\u2010stained tumorigenic BP1\u2010E cells with the microcell\u2010mediated chromosome transfer of normal chromosomes 11 and 17 was carried out.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A tendency of DNA amount distribution and nuclear size restoration to values typical of non\u2010transformed MCF\u201010F cells was demonstrated, especially after the transfer of chromosome 17. No reversion in chromatin texture was found after the transfer of chromosome 11 or 17.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Although the presence of a normal chromosome 17 should be considered among the necessary steps for tumorigenic human breast epithelial cells to recover their normality, a more complex genome balance is required for the entire nuclear chromatin of these cells to recover its totally normal supra\u2010organization and expression. Cytometry Part A 52A:70\u201376, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.a.10029","type":"journal-article","created":{"date-parts":[[2003,3,20]],"date-time":"2003-03-20T12:00:59Z","timestamp":1048161659000},"page":"70-76","source":"Crossref","is-referenced-by-count":11,"title":["DNA content, chromatin texture and nuclear morphology in benzo[a]pyrene\u2010transformed human breast epithelial cells after microcell\u2010mediated transfer of chromosomes 11 and 17"],"prefix":"10.1002","volume":"52A","author":[{"given":"Maria Luiza S.","family":"Mello","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benedicto","family":"de Campos Vidal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed H.","family":"Lareef","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun F.","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqi","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"Russo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,3,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/14.3.483"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4171"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/mc.2940090109"},{"key":"e_1_2_6_5_2","first-page":"963","article-title":"Activation of c\u2010myc, c\u2010neu, and int\u20102 oncogenes in the transformation of HBEC MCF\u201010F treated with chemical carcinogens in vitro","volume":"6","author":"Zhang PL","year":"1995","journal-title":"Int J Oncol"},{"key":"e_1_2_6_6_2","first-page":"41","article-title":"Microsatellite instability of D17S513 on chromosome 17 is associated with progression of breast cancer","volume":"11","author":"Yang X","year":"1997","journal-title":"Int J Oncol"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/18.5.1069"},{"key":"e_1_2_6_8_2","first-page":"629","article-title":"Functional roles of chromosomes 11 and 17 in the transformation of human breast epithelial cells in vitro","volume":"15","author":"Yang X","year":"1999","journal-title":"Int J Oncol"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2744(199902)24:2<118::AID-MC6>3.0.CO;2-L"},{"key":"e_1_2_6_10_2","first-page":"29","article-title":"Cellular and molecular changes induced by the chemical carcinogen Benz[a]pyrene in human breast epithelial cells and its association with smoking and breast cancer","volume":"3","author":"Russo IH","year":"2001","journal-title":"J Womens Cancer"},{"key":"e_1_2_6_11_2","first-page":"6063","article-title":"Microsatellite instability, mismatch repair deficiency, and genetic defects in human cancer cell lines","volume":"55","author":"Boyer JC","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5261.557"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0921-8777(96)00046-8"},{"key":"e_1_2_6_14_2","first-page":"241","article-title":"Microsatellite instability is correlated with lymph node\u2010positive breast cancer","volume":"3","author":"DeMarchis L","year":"1997","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000055921"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(96)04414-X"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006315315060"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006167210269"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/1520-6866(2000)20:5<283::AID-TCM4>3.0.CO;2-Y"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006490622103"},{"key":"e_1_2_6_21_2","first-page":"895","article-title":"Chromosome 17 (p13.1) transfer reverts transformation phenotypes in human breast epithelial cells","volume":"42","author":"Lareef MH","year":"2001","journal-title":"Proc Am Assoc Cancer Res"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.2741\/A335"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1995.1328"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01463655"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1989.tb02911.x"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00368153"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1155\/1999\/903790"},{"key":"e_1_2_6_28_2","unstructured":"NCBI. 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