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A high\u2010resolution contig array was developed specifically for chromosome 8q because this chromosome arm is frequently altered in many human cancers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A minimal tiling path contig of 702 8q\u2010specific bacterial artificial chromosome (BAC) clones was generated with a novel computational tool (BAC Contig Assembler). BAC clones were amplified by degenerative oligonucleotide primer (DOP) polymerase chain reaction and subsequently printed onto glass slides. For validation of the array DNA samples of gastroesophageal and prostate cancer cell lines, and chronic myeloid leukemia specimens were used, which were previously characterized by multicolor fluorescence in situ hybridization and conventional CGH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Single and double copy gains were confidently demonstrated with the 8q array. Single copy loss and high\u2010level amplifications were accurately detected and confirmed by bicolor fluorescence in situ hybridization experiments. The 8q array was further tested with paraffin\u2010embedded prostate cancer specimens. In these archival specimens, the copy number changes were confirmed. In fresh and archival samples, additional alterations were disclosed. In comparison with conventional CGH, the resolution of the detected changes was much improved, which was demonstrated by an amplicon of 0.7 Mb and a deletion of 0.6 Mb, both spanned by only six BAC clones.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A comprehensive array is presented, which provides a high\u2010resolution method for mapping copy number alterations on chromosome 8q. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.a.20102","type":"journal-article","created":{"date-parts":[[2004,12,16]],"date-time":"2004-12-16T16:16:14Z","timestamp":1103213774000},"page":"10-19","source":"Crossref","is-referenced-by-count":12,"title":["Construction and application of a full\u2010coverage, high\u2010resolution, human chromosome 8q genomic microarray for comparative genomic hybridization"],"prefix":"10.1002","volume":"63A","author":[{"given":"Mark","family":"van Duin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald","family":"van Marion","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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