{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T16:34:53Z","timestamp":1757781293082},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,2,14]],"date-time":"2006-02-14T00:00:00Z","timestamp":1139875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We investigate whether optical imaging can reliably detect abnormalities in tissue, in a range of specimens (live cells in vitro; fixed, fresh ex\u2010vivo and in vivo tissue), without the use of added contrast agents, and review our promising spectral methods for achieving quantitative, real\u2010time, high resolution intrasurgical optical diagnostics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We use reflectance, fluorescence, two\u2010photon, and Mie scattering imaging, performed with instrumentation we developed or modified, to detect intrinsic tissue signatures. Emphasis is on spectral\/hyperspectral imaging approaches allowing the equivalent of in vivo pathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>With experimental focus on unstained specimens, we demonstrate the ability to segment tissue images for cancer detection. Spectral reflectance imaging, coupled with advanced analysis, typically yields 90% specificity and sensitivity. Autofluorescence is also shown to be diagnostically useful, with lymph nodes results highlighted here. Elastic scattering hyperspectral imaging endoscopy, using a new instrument we designed and built, shows promise in bronchoscopic detection of dysplasia and early cancer in patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The results demonstrate that <jats:italic>advanced<\/jats:italic> optical imaging can detect and localize cellular signatures of cancer in real\u2010time, in vivo, without the use of contrast agents, in animals and humans. This is an important step towards tight spatio\u2010temporal coupling between such detection and clinical intervention. \u00a9 2006 International Society for Analytical Cytology<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.a.20220","type":"journal-article","created":{"date-parts":[[2006,2,14]],"date-time":"2006-02-14T23:46:37Z","timestamp":1139960797000},"page":"142-146","source":"Crossref","is-referenced-by-count":29,"title":["In vivo cytometry: A spectrum of possibilities"],"prefix":"10.1002","volume":"69A","author":[{"given":"Alice","family":"Chung","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Karlan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik","family":"Lindsley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastian","family":"Wachsmann\u2010Hogiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel L.","family":"Farkas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,2,14]]},"reference":[{"key":"e_1_2_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cursur.2004.12.011"},{"key":"e_1_2_4_3_2","doi-asserted-by":"publisher","DOI":"10.1088\/0031-9155\/45\/2\/201"},{"key":"e_1_2_4_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt892"},{"key":"e_1_2_4_5_2","doi-asserted-by":"publisher","DOI":"10.1118\/1.1418726"},{"key":"e_1_2_4_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt899"},{"key":"e_1_2_4_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/bp970088t"},{"key":"e_1_2_4_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1011-1344(03)00025-3"},{"key":"e_1_2_4_9_2","first-page":"479","article-title":"Autofluorescence spectroscopy of malpighian epithelial cells, as a new tool for analysis of cervical cancer precursors","volume":"18","author":"Millot C","year":"2003","journal-title":"Histol Histopathol"},{"key":"e_1_2_4_10_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2003.040709"},{"key":"e_1_2_4_11_2","doi-asserted-by":"publisher","DOI":"10.3109\/00016489709117795"},{"key":"e_1_2_4_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/1361-6374(199812)6:4<151::AID-BIO1>3.0.CO;2-G"},{"key":"e_1_2_4_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9101(2000)26:5<441::AID-LSM3>3.0.CO;2-0"},{"key":"e_1_2_4_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(01)00405-0"},{"key":"e_1_2_4_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02524348"},{"key":"e_1_2_4_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-7513-2_20"},{"key":"e_1_2_4_17_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0749.2001.140102.x"},{"key":"e_1_2_4_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(97)78155-4"},{"key":"e_1_2_4_19_2","doi-asserted-by":"publisher","DOI":"10.1088\/0031-9155\/42\/5\/005"},{"key":"e_1_2_4_20_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2001.24842"},{"key":"e_1_2_4_21_2","first-page":"75","article-title":"The hyperspectral imaging endoscope: a new tool for in vivo cancer detection","volume":"5","author":"Lindsley E","year":"2004","journal-title":"Prog Biomed Opt Imaging"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.a.20220","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.20220","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T13:33:22Z","timestamp":1696944802000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.20220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,2,14]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["10.1002\/cyto.a.20220"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.20220","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,2,14]]}}}