{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T23:59:30Z","timestamp":1778975970810,"version":"3.51.4"},"reference-count":12,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,4,27]],"date-time":"2006-04-27T00:00:00Z","timestamp":1146096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>Cell cycle analysis with flow cytometry using propidium iodide (PI) can be difficult in some cases because of the cell debris. Here, we introduce <jats:italic>d<\/jats:italic>ebris <jats:italic>r<\/jats:italic>emoval using <jats:italic>i<\/jats:italic>ntranuclear <jats:italic>p<\/jats:italic>rotein <jats:italic>s<\/jats:italic>taining (DRIPS), a novel method for separating intact nuclei and cell debris to different populations using carboxy\u2010fluorescein diacetate succinimidyl ester (CFSE).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>To study the apoptosis\u2010sensitivity, chicken DT40 B cell lymphoma cell line was \u03b3 irradiated. After the irradiation, the cells were incubated up to 8 h and the stages of the cell cycle were followed with flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>CFSE staining, done simultaneously with PI, stained the cell debris brighter than intact nuclei and could be excluded from the histogram with a simple gating procedure. The method is reliable and reproducible and can be executed within 15 min.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>DRIPS\u2010method greatly enhances the analysis of difficult cell cycle samples. \u00a9 2006 International Society for Analytical Cytology<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.a.20261","type":"journal-article","created":{"date-parts":[[2006,4,27]],"date-time":"2006-04-27T20:28:27Z","timestamp":1146169707000},"page":"552-554","source":"Crossref","is-referenced-by-count":5,"title":["Novel method for cell debris removal in the flow cytometric cell cycle analysis using carboxy\u2010fluorescein diacetate succinimidyl ester"],"prefix":"10.1002","volume":"69A","author":[{"given":"Perttu","family":"Terho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olli","family":"Lassila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,4,27]]},"reference":[{"key":"e_1_2_4_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19970101)27:1<1::AID-CYTO2>3.0.CO;2-L"},{"key":"e_1_2_4_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19980815)34:4<187::AID-CYTO3>3.0.CO;2-D"},{"key":"e_1_2_4_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990120203"},{"key":"e_1_2_4_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19970620)74:3<260::AID-IJC5>3.0.CO;2-X"},{"key":"e_1_2_4_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19970215)30:1<54::AID-CYTO8>3.0.CO;2-F"},{"key":"e_1_2_4_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19981201)33:4<385::AID-CYTO1>3.0.CO;2-5"},{"key":"e_1_2_4_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.1999.00877.x"},{"key":"e_1_2_4_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)90236-4"},{"key":"e_1_2_4_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0320(20000701)40:3<230::AID-CYTO8>3.0.CO;2-E"},{"key":"e_1_2_4_11_2","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.4049\/jimmunol.163.3.1133","article-title":"CD28, IL\u20102\u2010independent costimulatory pathways for CD8 T lymphocyte activation","volume":"163","author":"Sepulveda H","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_4_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(85)90312-5"},{"key":"e_1_2_4_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.1999.0744"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.a.20261","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.20261","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:22:50Z","timestamp":1697502170000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.20261"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4,27]]},"references-count":12,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,6]]}},"alternative-id":["10.1002\/cyto.a.20261"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.20261","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,4,27]]}}}