{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:05:30Z","timestamp":1758816330774},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T00:00:00Z","timestamp":1173139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background:<\/jats:title><jats:p>The analysis and isolation of high numbers of chromosomes smaller than 3 Mb in size (microchromosomes) with good purity is dependent primarily on the detection sensitivity of the flow cytometer and the precision of the sort unit. The aim of this study was to investigate the capability of using a conventional flow cytometer for the detection and sorting at high purity microchromosomes with an estimated size of 2.7 Mb.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods:<\/jats:title><jats:p>Chromosomes were isolated from a human cell line containing a pair of X\u2010derived microchromosomes, using a modified polyamine isolation buffer. The chromosome preparation was labeled with Hoechst and Chromomycin and analyzed and purified using a MoFlo sorter (DAKO) configured for high\u2010speed sorting. The purity of the flow\u2010sorted microchromosomes was assessed by reverse chromosome painting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results:<\/jats:title><jats:p>Improved resolution of the peak of microchromosomes in a bivariate plot of Hoechst versus Chromomycin fluorescence was obtainable after discriminating clumps and debris based on gating data within a FSC versus pulse width plot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions:<\/jats:title><jats:p>Chromosomes of smaller size, less than 3 Mb, can be detected with high resolution and flow\u2010sorted with high purity using a conventional flow sorter. \u00a9 2007 International Society for Analytical Cytology<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.a.20394","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T22:38:59Z","timestamp":1173220739000},"page":"410-413","source":"Crossref","is-referenced-by-count":8,"title":["Flow analysis and sorting of microchromosomes (&lt;3 Mb)"],"prefix":"10.1002","volume":"71A","author":[{"given":"Bee L.","family":"Ng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fengtang","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nigel P.","family":"Carter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,3,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00286654"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1320550122"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000015449"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10577-006-1029-6"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00351115"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.28.2.92"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90131-T"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19990201)35:2<129::AID-CYTO4>3.0.CO;2-A"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0497-345"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb00228.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/8.8.1417"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0694-586"},{"key":"e_1_2_6_14_2","first-page":"7","article-title":"Mammalian artificial chromosomes: A new tool for gene therapy","volume":"1","author":"Huxley C","year":"1994","journal-title":"Gene Ther"},{"key":"e_1_2_6_15_2","first-page":"125","article-title":"Satellite DNA\u2010based artificial chromosomes for use in gene therapy","volume":"3","author":"Hadlaczky G","year":"2001","journal-title":"Curr Opin Mol Ther"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20330"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/8.5.751"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1292-275"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0495-369"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.29.5.299"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0320(20010601)44:2<100::AID-CYTO1087>3.0.CO;2-Q"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.22-3-324"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009206926548"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbaexp.2003.12.003"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.a.20394","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.20394","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T18:44:52Z","timestamp":1697049892000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.20394"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3,6]]},"references-count":23,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2007,6]]}},"alternative-id":["10.1002\/cyto.a.20394"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.20394","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,3,6]]}}}