{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:15:34Z","timestamp":1791252934931,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,12,7]],"date-time":"2007-12-07T00:00:00Z","timestamp":1196985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Cryptosporidium parvum<\/jats:italic> is transmitted through water and can cause severe diarrhea. The diagnosis is usually based upon observer\u2010dependent microscopic detection of oocysts, with rather low sensitivity and specificity. Our objective was to optimize a flow cytometric (FC) protocol for the detection of <jats:italic>C. parvum<\/jats:italic>. A specific monoclonal antibody conjugated with R\u2010phycoerythrin was incubated with dead oocysts to determine the optimal antibody concentration. Serial concentrations of oocysts were stained with the optimized concentration and analyzed by FC. The lower detection limit was determined, and the possibility of cross\u2010reaction was investigated using prokaryotic and eukaryotic microorganisms. A FC protocol was optimized to detect oocysts in spiked human stools. The optimal antibody concentration was found to be 3.0 \u03bcg\/ml. The lowest number detectable was 2 \u00d7 10<jats:sup>3<\/jats:sup> oocysts\/ml. Staining procedure was specific, as no cross\u2010reactions were observed. This reliable and easy FC protocol allow the specific detection of <jats:italic>Cryptosporidium<\/jats:italic> oocysts, even at very low concentrations, which is important for public health and further studies of treatment efficacy. \u00a9 2007 International Society for Analytical Cytology.<\/jats:p>","DOI":"10.1002\/cyto.a.20502","type":"journal-article","created":{"date-parts":[[2007,12,7]],"date-time":"2007-12-07T17:17:11Z","timestamp":1197047831000},"page":"44-47","source":"Crossref","is-referenced-by-count":26,"title":["A flow cytometric protocol for detection of <i>Cryptosporidium<\/i> spp."],"prefix":"10.1002","volume":"73A","author":[{"given":"Joana M. M.","family":"Barbosa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Costa\u2010de\u2010Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ac\u00e1cio G.","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Hanscheid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard","family":"Shapiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cid\u00e1lia","family":"Pina\u2010Vaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,12,7]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.51.2.138"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.35.8.2013-2017.1997"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2005.02737.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0806.010271"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10661-005-1608-6"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.2.980-987.2004"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199503303321304"},{"key":"e_1_2_6_9_2","volume-title":"Cryptosporidium 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