{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T11:00:32Z","timestamp":1777546832943,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,10,3]],"date-time":"2008-10-03T00:00:00Z","timestamp":1222992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Multiparameter flow cytometry has become an essential tool for monitoring response to therapy in hematological malignancies, including B\u2010cell chronic lymphoproliferative disorders (B\u2010CLPD). However, depending on the expertise of the operator minimal residual disease (MRD) can be misidentified, given that data analysis is based on the definition of expert\u2010based bidimensional plots, where an operator selects the subpopulations of interest. Here, we propose and evaluate a probabilistic approach based on pattern classification tools and the Bayes theorem, for automated analysis of flow cytometry data from a group of 50 B\u2010CLPD versus normal peripheral blood B\u2010cells under MRD conditions, with the aim of reducing operator\u2010associated subjectivity. The proposed approach provided a tool for MRD detection in B\u2010CLPD by flow cytometry with a sensitivity of \u22648 \u00d7 10<jats:sup>\u22125<\/jats:sup> (median of \u22642 \u00d7 10<jats:sup>\u22127<\/jats:sup>). Furthermore, in 86% of B\u2010CLPD cases tested, no events corresponding to normal B\u2010cells were wrongly identified as belonging to the neoplastic B\u2010cell population at a level of \u226410<jats:sup>\u22127<\/jats:sup>. Thus, this approach based on the search for minimal numbers of neoplastic B\u2010cells similar to those detected at diagnosis could potentially be applied with both a high sensitivity and specificity to investigate for the presence of MRD in virtually all B\u2010CLPD. Further studies evaluating its efficiency in larger series of patients, where reactive conditions and non\u2010neoplastic disorders are also included, are required to confirm these results. \u00a9 2008 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.20638","type":"journal-article","created":{"date-parts":[[2008,10,3]],"date-time":"2008-10-03T22:16:15Z","timestamp":1223072175000},"page":"1141-1150","source":"Crossref","is-referenced-by-count":56,"title":["A probabilistic approach for the evaluation of minimal residual disease by multiparameter flow cytometry in leukemic B\u2010cell chronic lymphoproliferative disorders"],"prefix":"10.1002","volume":"73A","author":[{"given":"C.E.","family":"Pedreira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.S.","family":"Costa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Almeida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Fernandez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Quijano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Flores","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Barrena","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q.","family":"Lecrevisse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.J.M.","family":"Van Dongen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Orfao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,10,3]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20365"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20083"},{"key":"e_1_2_5_4_2","volume-title":"H43\u2010A2 Clinical Flow Cytometric Analysis of Neoplastic Hematolymphoid Cells; 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