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We isolated cancer cells with or without micronuclei to perform genomic analysis. Cell suspensions of HT1080 fibrosarcoma cells from either 2D culture or after isolation from 3D collagen matrix culture were stained with Hoechst 33342 and after classification for presence or absence of a micronucleus via bright\u2010field and epifluorescence microscopy, cells were individually aspirated with a micropipette. Subsequently, whole\u2010genome amplification and single\u2010cell comparative genomic hybridization (CGH) were applied to detect genomic aberrations. The data show a high\u2010fidelity isolation and genome amplification that lacks adverse effects by prior Hoechst 33342 staining. HT1080 cells showed a high degree of divergent amplifications, but neither location nor frequency of aberrations was dependent on 2D or 3D culture conditions or micronucleation. Thus, single\u2010cell selection of defined nuclear states is amenable to single\u2010cell CGH and here provides first insight into the aberration drift and genomic diversity in cancer cells with and without micronuclei. \u00a9 2009 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.20733","type":"journal-article","created":{"date-parts":[[2009,4,29]],"date-time":"2009-04-29T16:37:14Z","timestamp":1241023034000},"page":"562-568","source":"Crossref","is-referenced-by-count":18,"title":["Genomic instability of micronucleated cells revealed by single\u2010cell comparative genomic hybridization"],"prefix":"10.1002","volume":"75A","author":[{"given":"Andrea","family":"Imle","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Polzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Alexander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph A.","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Friedl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,4,29]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81683-9"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/35101065"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200801030"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2007.135004"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1768"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0962-8924(99)01661-X"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.8.4494"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)09838-0"},{"key":"e_1_2_5_10_2","first-page":"657","article-title":"Pathogenetic mechanisms of nuclear pleomorphism of tumour cells based on the mutator phenotype theory of carcinogenesis","volume":"18","author":"Bignold LP","year":"2003","journal-title":"Histol Histopathol"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(00)00065-8"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0027-5107(02)00086-6"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2280(2000)35:3<167::AID-EM3>3.0.CO;2-G"},{"key":"e_1_2_5_14_2","first-page":"77","article-title":"Reconstructing leukocyte migration in 3D extracellular matrix by time\u2010lapse videomicroscopy and computer\u2010assisted tracking","volume":"239","author":"Friedl P","year":"2004","journal-title":"Methods Mol Biol"},{"key":"e_1_2_5_15_2","doi-asserted-by":"crossref","first-page":"4973","DOI":"10.4049\/jimmunol.154.10.4973","article-title":"T lymphocyte locomotion in a three\u2010dimensional collagen matrix. 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