{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T16:22:17Z","timestamp":1788884537090,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2010,9,24]],"date-time":"2010-09-24T00:00:00Z","timestamp":1285286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Plasmodium falciparum<\/jats:italic> genotyping has recently undergone a revolution, and genome\u2010wide genotype datasets are now being collected for large numbers of parasite isolates. By contrast, phenotyping technologies have lagged behind, with few high throughput phenotyping platforms available. Invasion of human erythrocytes by <jats:italic>Plasmodium falciparum<\/jats:italic> is a phenotype of particular interest because of its central role in parasite development. Invasion is a variable phenotype influenced by natural genetic variation in both the parasite and host and is governed by multiple overlapping and in some instances redundant parasite\u2013erythrocyte interactions. To facilitate the scale\u2010up of erythrocyte invasion phenotyping, we have developed a novel platform based on two\u2010color flow cytometry that distinguishes parasite invasion from parasite growth. Target cells that had one or more receptors removed using enzymatic treatment were prelabeled with intracellular dyes CFDA\u2010SE or DDAO\u2010SE, incubated with <jats:italic>P. falciparum<\/jats:italic> parasites, and parasites that had invaded either labeled or unlabeled cells were detected with fluorescent DNA\u2010intercalating dyes Hoechst 33342 or SYBR Green I. Neither cell label interfered with erythrocyte invasion, and the combination of cell and parasite dyes recapitulated known invasion phenotypes for three standard laboratory strains. Three different dye combinations with minimal overlap have been validated, meaning the same assay can be adapted to instruments harboring several different combinations of laser lines. The assay is sensitive, operates in a 96\u2010well format, and can be used to quantitate the impact of natural or experimental genetic variation on erythrocyte invasion efficiency. \u00a9 2010 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.20972","type":"journal-article","created":{"date-parts":[[2010,9,24]],"date-time":"2010-09-24T20:11:05Z","timestamp":1285359065000},"page":"1067-1074","source":"Crossref","is-referenced-by-count":78,"title":["An adaptable two\u2010color flow cytometric assay to quantitate the invasion of erythrocytes by <i>Plasmodium falciparum<\/i> parasites"],"prefix":"10.1002","volume":"77A","author":[{"given":"Michel","family":"Theron","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard L.","family":"Hesketh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sathish","family":"Subramanian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian C.","family":"Rayner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,9,24]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.006"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm807"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/649902"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2007.01.007"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00122-07"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1856-1863.2003"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.5886-5891.2004"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2006.05.011"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.11.5784-5791.1999"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-2-r21"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.528"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20541"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2008.10.008"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1171085"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.13.7509"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg096"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.21642"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.1999.00877.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(99)00115-2"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.20856"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.5.1807-1810.2004"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.5.1803-1806.2004"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0010037"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2009.07.095"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.6.156"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.11.1571"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.a.20972","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.20972","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T15:26:24Z","timestamp":1700580384000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.20972"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,24]]},"references-count":26,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2010,11]]}},"alternative-id":["10.1002\/cyto.a.20972"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.20972","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,9,24]]}}}