{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T15:12:09Z","timestamp":1790521929598,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2011,6,21]],"date-time":"2011-06-21T00:00:00Z","timestamp":1308614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Following activation by antigen, T cells enter the cell cycle in stages that can be defined with flow cytometric markers. We show that these markers include the increase of forward light scatter width (FSC\u2010W) signal and the ratio of FSC area\/peak. This change in light scatter precedes the first cell division and may reflect blast transformation. We show that the FSC\u2010W parameter can be used, alone or in combination with other activation markers, to monitor the relative and absolute numbers of T cells responding to a proliferative stimulus. In contrast to dye dilution assays, the FSC\u2010W method does not allow discrimination between consecutive cell divisions, but it has several advantages and could complement the dye dilution assay. Our findings also show that the routine elimination of doublets based on FSC signals may exclude proliferating T cells from the analysis. \u00a9 2011 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.21096","type":"journal-article","created":{"date-parts":[[2011,6,21]],"date-time":"2011-06-21T15:31:29Z","timestamp":1308670289000},"page":"646-652","source":"Crossref","is-referenced-by-count":42,"title":["Forward light scatter is a simple measure of T\u2010cell activation and proliferation but is not universally suited for doublet discrimination"],"prefix":"10.1002","volume":"79A","author":[{"given":"Ralph M.","family":"B\u00f6hmer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Bandala\u2010Sanchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonard C.","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,6,21]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"263","article-title":"The use of tritiated thymidine in the study of DNS synthesis and cell turnover in hemopoietic tissues","volume":"8","author":"Cronkite EP","year":"1959","journal-title":"Lab Invest"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02339807"},{"key":"e_1_2_6_4_2","first-page":"235","article-title":"A comparison of mathematical methods for the analysis of DNA histograms obtained by flow cytometry","volume":"15","author":"Baisch H","year":"1982","journal-title":"Cell Tissue Kinet"},{"key":"e_1_2_6_5_2","first-page":"101","article-title":"Flow cytometric cell cycle analysis using the quenching of 33258 Hoechst fluorescence by bromodeoxyuridine incorporation","volume":"12","author":"Bohmer RM","year":"1979","journal-title":"Cell Tissue Kinet"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990020107"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.18.5573"},{"key":"e_1_2_6_8_2","first-page":"1","article-title":"Cell\u2010production rates estimated by the use of vincristine sulphate and flow cytometry. 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