{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T10:30:08Z","timestamp":1782297008313,"version":"3.54.5"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2011,8,16]],"date-time":"2011-08-16T00:00:00Z","timestamp":1313452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2011,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Conventional photothermal (PT) and photoacousic (PA) imaging, spectroscopy, and cytometry are preferentially based on positive PT\/PA effects, when signals are above background. Here, we introduce PT\/PA technique based on detection of negative signals below background. Among various new applications, we propose label\u2010free in vivo flow cytometry of circulating clots. No method has been developed for the early detection of clots of different compositions as a source of thromboembolism including ischemia at strokes and myocardial infarction. When a low\u2010absorbing, platelet\u2010rich clot passes a laser\u2010irradiated vessel volume, a transient decrease in local absorption results in an ultrasharp negative PA hole in blood background. Using this phenomenon alone or in combination with positive contrasts, we demonstrated identification of white, red, and mixed clots on a mouse model of myocardial infarction and human blood. The concentration and size of clots were measured with threshold down to few clots in the entire circulation with size as low as 20 \u03bcm. This multiparameter diagnostic platform using portable personal high\u2010speed flow cytometer with negative dynamic contrast mode has potential to real\u2010time defining risk factors for cardiovascular diseases, and for prognosis and prevention of stroke or use clot count as a marker of therapy efficacy. Possibility for label\u2010free detection of platelets, leukocytes, tumor cells or targeting themby negative PA probes (e.g., nonabsorbing beads or bubbles) is also highlighted. \u00a9 2011 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.21106","type":"journal-article","created":{"date-parts":[[2011,8,16]],"date-time":"2011-08-16T15:45:49Z","timestamp":1313509549000},"page":"814-824","source":"Crossref","is-referenced-by-count":39,"title":["In vivo flow cytometry of circulating clots using negative photothermal and photoacoustic contrasts"],"prefix":"10.1002","volume":"79A","author":[{"given":"Ekaterina I.","family":"Galanzha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mustafa","family":"Sarimollaoglu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry 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