{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,9]],"date-time":"2024-06-09T13:33:13Z","timestamp":1717939993503},"reference-count":43,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2011,7,22]],"date-time":"2011-07-22T00:00:00Z","timestamp":1311292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>While previous studies have investigated the utility of Luminex technology in comparison to other standard techniques, there have been few studies directly comparing different bead\u2010based assays. A key barrier to establishing Luminex technology in research or clinical laboratories is the apparent need to purchase not only encoded bead sets but also the Luminex instrument. However, as flow cytometry instrumentation continues to improve in sensitivity and in the number and diversity of detection parameters, a diverse range of bead\u2010based assays is likely to emerge. Human papillomavirus (HPV) genotyping requires multiplexed analysis of 10\u2013100 individual genotypes per sample, which is well suited to bead\u2010based assays whilst technically challenging and costly for related technologies (e.g., qPCR). Here we performed an unbiased technical comparison between Luminex technology and our in\u2010house 3\u2010mercaptopropyl trimethoxysilane (\u201cMPS\u201d) bead platform, which has been designed for integration with generic cytometry instruments. In genotyping 200 clinical samples, we compared the two bead assays against the goldstandard Roche Line Blot (RLB) assay, and both performed well in receiver\u2010operator characteristic (ROC) curve analysis. We also show instrument\u2010based differences are a significant factor in comparing the methods, which needs to be considered in future comparative studies. These multi\u2010platform analyses are important in establishing the validity of new methods, as well as highlighting specific advantages anddisadvantages of the assays for specific applications. \u00a9 2011 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.21109","type":"journal-article","created":{"date-parts":[[2011,7,25]],"date-time":"2011-07-25T14:47:10Z","timestamp":1311605230000},"page":"713-719","source":"Crossref","is-referenced-by-count":5,"title":["Multiplatform comparison of multiplexed bead arrays using HPV genotyping as a test case"],"prefix":"10.1002","volume":"79A","author":[{"given":"Simon 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