{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:43:08Z","timestamp":1791308588186,"version":"4.3.1"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,6,6]],"date-time":"2012-06-06T00:00:00Z","timestamp":1338940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diagnosis of blastic plasmacytoid dendritic cell neoplasm (BPDCN) or plasmacytoid dendritic cell leukemia (pDCL) is mainly based on immunophenotypical characterization of leukemic cells in blood or bone marrow samples. We tested by flow cytometry intracellular expression of the proto\u2010oncogene T\u2010cell leukemia 1 (TCL1), as well as membrane and intracellular expression of immunoglobulin\u2010like transcript 7 (ILT7) in 21 pDCL samples and 61 non\u2010pDC acute leukemia samples [i.e., 14 B\u2010acute lymphoblastic leukemia (B\u2010ALL), 9 T\u2010ALL and 38 acute myeloid leukemia (AML)]. TCL1 is highly expressed in all pDCL samples while at a statistically lower level in all B\u2010ALL and 34% of AML. Statistical analysis shows that intensity of TCL1 expression is a good marker for differential diagnosis of pDCL versus other acute leukemia (area under the receiver\u2010operating characteristic curve, [AUC]: 0.96). By contrast, ILT7 positivity is limited to few pDCL samples and cannot be useful for diagnosis purpose. In conclusion, high intracellular intensity of TCL1 expression is currently the best marker for pDC lineage assignment by flow cytometry, which is particularly useful to distinguish pDCL from CD4<jats:sup>+<\/jats:sup> CD56<jats:sup>+\/\u2212<\/jats:sup> undifferentiated or monoblastic acute leukemia. Thus, intracellular TCL1 detection should be included in acute leukemia diagnosis panels used in hematology laboratories. \u00a9 2012 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.22072","type":"journal-article","created":{"date-parts":[[2012,6,6]],"date-time":"2012-06-06T15:08:12Z","timestamp":1338995292000},"page":"718-724","source":"Crossref","is-referenced-by-count":22,"title":["Intracytoplasmic detection of TCL1\u2014but not ILT7\u2014by flow cytometry is useful for blastic plasmacytoid dendritic cell leukemia diagnosis"],"prefix":"10.1002","volume":"81A","author":[{"given":"Fanny","family":"Angelot\u2010Delettre","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabeha","family":"Biichle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Ferrand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle","family":"Seilles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B\u00e9atrice","family":"Gaugler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronique","family":"Harrivel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Alessandra","family":"Rosenthal\u2010Allieri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Deconinck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Saas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francine","family":"Garnache\u2010Ottou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,6,6]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-03-209262"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.5.1556"},{"key":"e_1_2_5_4_2","first-page":"1873","article-title":"Blastic plasmacytoid dendritic cell neoplasm in children: Diagnostic features and clinical implications","volume":"95","author":"Jegalian AG","journal-title":"Haematologica 2010;"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1704215"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-10-117531"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-04-018143"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2008.359"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2006.06458.x"},{"key":"e_1_2_5_10_2","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1097\/PAS.0b013e3181c5e26b","article-title":"Cutaneous manifestations of blastic plasmacytoid dendritic cell neoplasm\u2010morphologic and phenotypic variability in a series of 33 patients","volume":"34","author":"Cota C","journal-title":"Am J Surg Pathol"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2009.07679.x"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-06-2416"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1309\/0QPPAVTUPCV9UCLV"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204596"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-10-3297"},{"key":"e_1_2_5_16_2","first-page":"2095","article-title":"Regulation of TCL1 expression in B\u2010 and T\u2010cell lymphomas and reactive lymphoid tissues","volume":"60","author":"Narducci MG","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2004.02.018"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.09.016"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni0506-441"},{"key":"e_1_2_5_20_2","first-page":"1783","article-title":"Proposals for the immunological classification of acute leukemias. 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