{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T14:33:29Z","timestamp":1778078009000,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,3,15]],"date-time":"2013-03-15T00:00:00Z","timestamp":1363305600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We present measurements by deep\u2010ultraviolet mass mapping of nucleic acid (NA) and protein for five commonly cultured and three primary cell types. The dry mass distribution at submicron resolution was determined on a single\u2010cell basis for 250\u2013500 cells from each of these types. Since the method carries a direct reference to a spectrophotometric standard (molar extinction coefficient), we are able to calibrate the absolute weight distributions both on a cell\u2010to\u2010cell basis within each type and across types. We also provide a calibration in absolute mass units for fluorescence\u2010based measurements (flow cytometry and fluorescence microscopy). As might be expected the cultured cell lines show a high concentration of nucleic acids in the nuclear compartment, much larger than the genomic 2C number even in the G1 stage. The whole\u2010cell nucleic\u2010acid\/protein ratio was found to be a characteristic of cell lines that persists independent of cell cycle and, as a result, this ratio has some value for phenotyping. Primary chicken red blood cells (cRBC), often used as a cytometry standard, were determined to have a nuclear\u2010isolated nucleic acid content much closer to the genomic number than the cultured cell lines (cRBC: 3.00 pg total NA, 2.30 pg DNA, and 0.70 pg RNA). The individual blastomeres (<jats:italic>n<\/jats:italic> = 54) from mouse embryos at eight\u2010cell stage were measured and found to vary by more than a factor or two in total protein and nucleic acid content (0.8\u20132.3 ng total protein, 70\u2013150 pg total NA). The ratio of nucleic acid to protein was more nearly constant for each blastomere from a particular embryo and this ratio was found to be an identifying characteristic that varies from embryo to embryo obtained from a single flushing of a mouse. \u00a9 2013 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.22277","type":"journal-article","created":{"date-parts":[[2013,3,15]],"date-time":"2013-03-15T23:10:28Z","timestamp":1363389028000},"page":"540-551","source":"Crossref","is-referenced-by-count":60,"title":["Intracellular protein and nucleic acid measured in eight cell types using deep\u2010ultraviolet mass mapping"],"prefix":"10.1002","volume":"83A","author":[{"given":"Man C.","family":"Cheung","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"LaCroix","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian K.","family":"McKenna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Winkelman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel J.","family":"Ehrlich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,3,15]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004180050367"},{"key":"e_1_2_5_3_2","series-title":"Methods in Molecular Biology","first-page":"163","volume-title":"Drosophila Cytogenetic Protocols","author":"Rasch 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measurements","volume":"37","author":"McHowat J","year":"1996","journal-title":"J Lipid Res"},{"key":"e_1_2_5_14_2","first-page":"113","article-title":"Dry mass, lipid content and protein content of the intact and zona\u2010free mouse ovum","volume":"12","author":"Loewenstein JE","year":"1964","journal-title":"J Embryol Exp 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