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In the present proof\u2010of\u2010concept work, we examined the potential of the fluorescent dye C11\u2010BODIPY<jats:sup>591\/581<\/jats:sup> to probe lipid oxidation in the green microalga <jats:italic>Chlamydomonas reinhardtii<\/jats:italic>. C11\u2010BODIPY<jats:sup>591\/581<\/jats:sup> staining was combined with flow cytometry measurements to obtain multiparameter information on cellular features and oxidative stress damage within single cells. First, staining conditions were optimized by exploring the capability of the dye to stain algal cells under increasing cell and dye concentrations and different staining procedures. Then lipid oxidation in algae induced by short\u2010 and long\u2010term exposures to the three metallic micropollutants, copper, mercury, and nanoparticulate copper oxide, and the two organic contaminants, diethyldithiocarbamate (DDC) and diuron was determined. In this work we pointed out C11\u2010BODIPY<jats:sup>591\/581<\/jats:sup> applicability in a wide range of exposure conditions, including studies of oxidation as a function of time and that it is suitable for in vivo measurements of lipid oxidation due to its high permeation and stability in cells and its low interference with algal autofluorescence. \u00a9 2013 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.22338","type":"journal-article","created":{"date-parts":[[2013,8,13]],"date-time":"2013-08-13T18:15:51Z","timestamp":1376417751000},"page":"952-961","source":"Crossref","is-referenced-by-count":59,"title":["Optimization of the C11\u2010BODIPY<sup>581\/591<\/sup> dye for the determination of lipid oxidation in <i>Chlamydomonas reinhardtii<\/i> by flow cytometry"],"prefix":"10.1002","volume":"83","author":[{"given":"Giulia","family":"Cheloni","sequence":"first","affiliation":[{"name":"Environmental Biogeochemistry and Ecotoxicology, Institute F.\u2010A. 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