{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:48:21Z","timestamp":1792000101537,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,8,6]],"date-time":"2014-08-06T00:00:00Z","timestamp":1407283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Immunophenotyping of blood lymphocyte subsets and activation markers is a basic tool in the diagnostic process of primary immunodeficiency diseases, its use becoming more and more widespread as the knowledge about these illnesses increases. However, the availability of reliable reference values, which need to be age\u2010matched for the pediatric population, is a pre\u2010requisite for the reliable interpretation of immunophenotyping data. Aim of this study is to analyze the lymphocyte subsets and activation markers distribution in children aged 0\u201318 years referring to the University Hospital of Padova and to create age\u2010matched reference values expressed by percentiles, thus providing a valuable guideline for the interpretation of the immunophenotype. \u00a9 2014 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.22520","type":"journal-article","created":{"date-parts":[[2014,8,6]],"date-time":"2014-08-06T20:03:09Z","timestamp":1407355389000},"page":"81-85","source":"Crossref","is-referenced-by-count":133,"title":["Lymphocytes subsets reference values in childhood"],"prefix":"10.1002","volume":"87","author":[{"given":"F.","family":"Tosato","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine University Hospital of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Bucciol","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology Oncology University Hospital of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Pantano","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University Hospital of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. C.","family":"Putti","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology Oncology University Hospital of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.C.","family":"Sanzari","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University Hospital of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Basso","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology Oncology University Hospital of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Plebani","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University Hospital of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,6]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2006.03138.x"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.10.013"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.08.043"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2003.07.003"},{"key":"e_1_2_4_6_1","first-page":"388","article-title":"Reference values for lymphocyte subpopulations in peripheral blood","volume":"130","author":"Comans\u2010Bitter WM","year":"1997","journal-title":"Adapted from J Pediatr"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.1860040305"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1992.tb12187.x"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/100.2.111"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01955518"},{"key":"e_1_2_4_11_1","first-page":"143","article-title":"T\u2010cell subpopulations in pediatric healthy children: age\u2010normal values","volume":"38","author":"Melaranci C","year":"1992","journal-title":"J Clin Lab Immunol"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1995.tb03735.x"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19960315)26:1<8::AID-CYTO2>3.0.CO;2-E"},{"key":"e_1_2_4_14_1","first-page":"518","article-title":"Age\u2010related changes in T\u2010 and B\u2010lymphocyte subpopulations in the peripheral blood","volume":"107","author":"Hicks MJ","year":"1983","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-198611000-00017"},{"key":"e_1_2_4_16_1","first-page":"175","article-title":"Flow cytometric analysis of lymphocyte subset phenotypes comparing normal children and adults","volume":"5","author":"Babcock GF","year":"1987","journal-title":"Diagn Clin Immunol"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1994.65"},{"key":"e_1_2_4_18_1","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1128\/CDLI.5.5.632-635.1998","article-title":"Age\u2010related changes in blood lymphocyte subsets of Saudi Arabian healthy children","volume":"5","author":"Shahabuddin S","year":"1998","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(05)80430-5"},{"key":"e_1_2_4_20_1","doi-asserted-by":"crossref","first-page":"1484","DOI":"10.1001\/jama.1992.03480110060034","article-title":"Lymphocyte subset in healthy children during the first 5 years of life","volume":"267","author":"Denny T","year":"1992","journal-title":"JAMA"},{"key":"e_1_2_4_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1994.1023"},{"key":"e_1_2_4_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19981015)34:5<235::AID-CYTO5>3.0.CO;2-0"},{"key":"e_1_2_4_23_1","first-page":"453","article-title":"The reference range of CD4+ and CD8+ T\u2010lymphocytes in healthy non\u2010infected infants born to HIV\u20101 seropositive mothers; a preliminary study at Siriraj Hospital","volume":"29","author":"Likanosakul S","year":"1998","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"e_1_2_4_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(97)70313-5"},{"key":"e_1_2_4_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01957900"},{"key":"e_1_2_4_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199211120-00006"},{"key":"e_1_2_4_27_1","doi-asserted-by":"publisher","DOI":"10.1159\/000101357"},{"key":"e_1_2_4_28_1","doi-asserted-by":"publisher","DOI":"10.3109\/10408368909106595"},{"key":"e_1_2_4_29_1","unstructured":"Clinical and Laboratory Standards Institute. Enumeration of Immunologically Defined Cell Populations by Flow Cytometry; Approved Guideline 2nd ed. CLSI document H42\u2010A2.Wayne PA:Clinical and Laboratory Standards Institute;2007."},{"key":"e_1_2_4_30_1","volume-title":"Defining, Establishing, and Verifying Reference Intervals in the Clinical Laboratory: Approved Guideline","author":"CLSI","year":"2008"}],"container-title":["Cytometry Part A"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.a.22520","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.a.22520","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T03:56:48Z","timestamp":1696651008000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.a.22520"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,6]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1002\/cyto.a.22520"],"URL":"https:\/\/doi.org\/10.1002\/cyto.a.22520","archive":["Portico"],"relation":{},"ISSN":["1552-4922","1552-4930"],"issn-type":[{"value":"1552-4922","type":"print"},{"value":"1552-4930","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,6]]}}}