{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T07:03:24Z","timestamp":1788505404976,"version":"build-2803163510"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,12,15]],"date-time":"2015-12-15T00:00:00Z","timestamp":1450137600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Abnormal DNA ploidy is a valuable prognostic factor in many neoplasms, especially in hematological neoplasms like B\u2010cell acute lymphoblastic leukemia (B\u2010ALL) and multiple myeloma (MM). Current methods of flow\u2010cytometric (FC) DNA\u2010ploidy evaluation are either technically difficult or limited to three\u2010 to four\u2010color immunophenotyping and hence, challenging to evaluate DNA\u2010ploidy in minute tumor population with background rich of its normal counterpart cells and other hematopoietic cells. We standardized a novel sensitive and easy method of simultaneous evaluation of six\u2010 to seven\u2010color immunophenotyping and DNA\u2010ploidy using a dye\u2013FxCycle Violet (FCV). Linearity, resolution, and coefficient of variation (CV) for FCV were studied using chicken erythrocyte nuclei. Ploidy results of FCV were compared with Propidium iodide (PI) in 20 samples and intra\u2010assay variation for FCV was studied. Using this six\u2010color immunophenotyping &amp; FCV\u2010protocol DNA\u2010ploidy was determined in bone\u2010marrow samples from 124 B\u2010ALL &amp; 50 MM patients. Dilution experiment was also conducted to determine the sensitivity in detection of aneuploidy in minute tumor population. FCV revealed high linearity and resolution in 450\/50 channel. On comparison with PI, CV of Go\/G1\u2010peak with FCV (mean\u2010CV 4.1%) was slightly higher than PI (mean\u2010CV 2.9%) but had complete agreement in ploidy results. Dilution experiment showed that aneuploidy could be accurately detected up to the limit of 0.01% tumor cells. Intra\u2010assay variation was very low with CV of 0.005%. In B\u2010ALL, hypodiploidy was noted in 4%, hyperdiploidy in 24%, near\u2010hyperdiploidy in 13% and remaining 59% were diploid. In MM, hypodiploidy was in 2%, hyperdiploidy in 58%, near\u2010hyperdiploidy in 8% and remaining 30% were diploid. FCV\u2010based DNA\u2010ploidy method is a sensitive and easy method for simultaneous evaluation of six\u2010color immunophenotyping and DNA analysis. It is useful in DNA\u2010ploidy evaluation of minute tumor population in cases like minimal residual disease and MM precursor conditions. \u00a9 2015 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.22803","type":"journal-article","created":{"date-parts":[[2015,12,15]],"date-time":"2015-12-15T13:12:32Z","timestamp":1450185152000},"page":"281-291","source":"Crossref","is-referenced-by-count":32,"title":["A novel and easy\n                    <scp>F<\/scp>\n                    xCycle\n                    <scp>\u2122<\/scp>\n                    violet based flow cytometric method for simultaneous assessment of\n                    <scp>DNA<\/scp>\n                    ploidy and six\u2010color immunophenotyping"],"prefix":"10.1002","volume":"89","author":[{"given":"Prashant","family":"Tembhare","sequence":"first","affiliation":[{"name":"Hematopathology Laboratory Tata Memorial Center Hospital Parel Mumbai 400012 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