{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T13:41:11Z","timestamp":1771508471048,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,8,30]],"date-time":"2016-08-30T00:00:00Z","timestamp":1472515200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Reactive oxygen species (ROS) are constantly produced in cells, mainly by mitochondria, as a consequence of aerobic respiration. Most ROS derive from superoxide, which is rapidly converted to hydrogen peroxide. ROS are involved in the regulation of several physiological and pathological processes, and the possibility to measure them simultaneously is needed, when the redox status of the cells is modified by experimental\/biological conditions. Flow cytometry is the main technology that generates multiple information at the single cell level in a high\u2010throughput manner, and gives rapid and quantitative measurements of different ROS with high sensitivity and reproducibility. Here, we describe a novel approach to detect simultaneously mitochondrial hydrogen peroxide and mitochondrial superoxide in living cells. The staining has been performed by using the fluorescent dyes MitoSOX Red Mitochondrial Superoxide Indicator, Mitochondria Peroxy Yellow 1, Annexin\u2010V Pacific Blue conjugate, TO\u2010PRO\u20103 iodide, anti\u2010CD4\u2010APC\u2010Cy7 and \u2010CD8\u2010Pacific Orange mAbs. We used this approach to quantify mitochondrial ROS in CD4+ and CD8+ T cells form patients affected by Down syndrome and age\u2010 and sex\u2010matched healthy donors. \u00a9 2016 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.22936","type":"journal-article","created":{"date-parts":[[2016,8,30]],"date-time":"2016-08-30T14:47:22Z","timestamp":1472568442000},"page":"1106-1110","source":"Crossref","is-referenced-by-count":17,"title":["Quantification of mitochondrial reactive oxygen species in living cells by using multi\u2010laser polychromatic flow cytometry"],"prefix":"10.1002","volume":"89","author":[{"given":"Sara","family":"De Biasi","sequence":"first","affiliation":[{"name":"Department of Surgery Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia Modena Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara","family":"Gibellini","sequence":"additional","affiliation":[{"name":"Department of 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Specialty Medicine, University of Bologna  Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Cossarizza","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences for Children and Adults University of Modena and Reggio Emilia Modena 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