{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:27:12Z","timestamp":1790771232418,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,5,19]],"date-time":"2017-05-19T00:00:00Z","timestamp":1495152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["260901"],"award-info":[{"award-number":["260901"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201010\u2010EQPX\u201004\u201301, ANR\u201011\u2010LABX\u20100024, ANR\u201014\u2010CE14\u20100024, ANR\u201014\u2010CE08\u20100017, ANR\u201014\u2010CE14\u20100027\u201301"],"award-info":[{"award-number":["ANR\u201010\u2010EQPX\u201004\u201301, ANR\u201011\u2010LABX\u20100024, ANR\u201014\u2010CE14\u20100024, ANR\u201014\u2010CE08\u20100017, ANR\u201014\u2010CE14\u20100027\u201301"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry Pt A"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tuberculosis (TB) is still a major global threat, killing more than one million persons each year. With the constant increase of <jats:italic>Mycobacterium tuberculosis<\/jats:italic> strains resistant to first\u2010 and second\u2010line drugs, there is an urgent need for the development of new drugs to control the propagation of TB. Although screenings of small molecules on axenic <jats:italic>M. tuberculosis<\/jats:italic> cultures were successful for the identification of novel putative anti\u2010TB drugs, new drugs in the development pipeline remains scarce. Host\u2010directed therapy may represent an alternative for drug development against TB. Indeed, <jats:italic>M. tuberculosis<\/jats:italic> has multiple specific interactions within host phagocytes, which may be targeted by small molecules. In order to enable drug discovery strategies against microbes residing within host macrophages, we developed multiple fluorescence\u2010based HT\/CS phenotypic assays monitoring the intracellular replication of <jats:italic>M. tuberculosis<\/jats:italic> as well as its intracellular trafficking. What we propose here is a population\u2010based, multi\u2010parametric analysis pipeline that can be used to monitor the intracellular fate of <jats:italic>M. tuberculosis<\/jats:italic> and the dynamics of cellular events such as phagosomal maturation (acidification and permeabilization), zinc poisoning system or lipid body accumulation. Such analysis allows the quantification of biological events considering the host\u2010pathogen interplay and may thus be derived to other intracellular pathogens. \u00a9 2017 International Society for Advancement of Cytometry<\/jats:p>","DOI":"10.1002\/cyto.a.23129","type":"journal-article","created":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T20:33:40Z","timestamp":1495571620000},"page":"983-994","source":"Crossref","is-referenced-by-count":22,"title":["Phenotypic assays for <i>Mycobacterium tuberculosis<\/i> infection"],"prefix":"10.1002","volume":"91","author":[{"given":"Ok\u2010Ryul","family":"Song","sequence":"first","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 8204 \u2010 CIIL \u2010 Centre d'Infection et d'Immunit\u00e9 de Lille F\u201059000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Deboosere","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 8204 \u2010 CIIL \u2010 Centre d'Infection et d'Immunit\u00e9 de Lille F\u201059000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Delorme","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 8204 \u2010 CIIL \u2010 Centre d'Infection et d'Immunit\u00e9 de Lille F\u201059000 France"},{"name":"Tuberculosis Research Laboratory, Institut Pasteur Korea Bundang\u2010gu, Seongnam\u2010si Gyeonggi\u2010do 13488 Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe J.","family":"Queval","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 8204 \u2010 CIIL \u2010 Centre d'Infection et d'Immunit\u00e9 de Lille F\u201059000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaspard","family":"Deloison","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 8204 \u2010 CIIL \u2010 Centre d'Infection et d'Immunit\u00e9 de Lille F\u201059000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth","family":"Werkmeister","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 8204 \u2010 CIIL \u2010 Centre d'Infection et d'Immunit\u00e9 de Lille F\u201059000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Lafont","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 8204 \u2010 CIIL \u2010 Centre d'Infection et d'Immunit\u00e9 de Lille F\u201059000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Baulard","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 8204 \u2010 CIIL \u2010 Centre d'Infection et d'Immunit\u00e9 de Lille F\u201059000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaella","family":"Iantomasi","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 8204 \u2010 CIIL \u2010 Centre d'Infection et d'Immunit\u00e9 de Lille F\u201059000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priscille","family":"Brodin","sequence":"additional","affiliation":[{"name":"University of Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, U1019 \u2010 UMR 8204 \u2010 CIIL \u2010 Centre d'Infection et d'Immunit\u00e9 de Lille F\u201059000 France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1781"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1906"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.11.014"},{"key":"e_1_2_6_5_1","unstructured":"WHO. 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